Tsai, Shengdar Q.

Efficient genome editing in zebrafish using a CRISPR-Cas system

Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology

CRISPR and Genetic EngineeringZebrafish Biomedical Research Applications

Cited by 2,937

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2,673

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2,268

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,624

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Tsai, S. Q., Wyvekens, N., Khayter, C., Foden, J. A., Thapar, V., Reyon, D., Goodwin, M. J., Aryee, M. J., & Joung, J. K. (2014) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 952

CIRCLE-Seq: A highly sensitive in vitro screen for genome-wide CRISPR-cas9 nuclease off-targets

Tsai, S. Q., Nguyen, N. T., Malagon-Lopez, J., Topkar, V. V., Aryee, M. J., & Joung, J. K. (2017) · Nature Methods

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 814

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

Kleinstiver, B. P., Tsai, S. Q., Prew, M. S., Nguyen, N. T., Welch, M. M., Lopez, J. M., McCaw, Z. R., Aryee, M. J., & Joung, J. K. (2016) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 670

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Topkar, V. V., Zheng, Z., & Joung, J. K. (2015) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 611

Highly efficient therapeutic gene editing of human hematopoietic stem cells

Wu, Y., Zeng, J., Roscoe, B. P., Liu, P., Yao, Q., Lazzarotto, C. R., Clement, K., Cole, M. A., Luk, K., Baricordi, C., et al. (2019) · Nature Medicine

Hemoglobinopathies and Related DisordersCRISPR and Genetic Engineering

Cited by 515

High levels of AAV vector integration into CRISPR-induced DNA breaks

Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., et al. (2019) · Nature Communications

Virus-based gene therapy researchCRISPR and Genetic Engineering

Cited by 431

Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity

Prinzing, B., Zebley, C. C., Petersen, C. T., Fan, Y., Anido, A. A., Yi, Z., Nguyen, P., Houke, H., Bell, M., Haydar, D., et al. (2021) · Science Translational Medicine

CAR-T cell therapy researchT-cell and B-cell Immunology

Cited by 349

Genome editing of HBG1 and HBG2 to induce fetal hemoglobin

Métais, J.-Y., Doerfler, P. A., Mayuranathan, T., Bauer, D. E., Fowler, S. C., Hsieh, M. M., Katta, V., Keriwala, S., Lazzarotto, C. R., Luk, K., et al. (2019) · Blood Advances

Hemoglobinopathies and Related DisordersCRISPR and Genetic Engineering

Cited by 177

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2