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Fluorescence Assay in Biology and Medicine
Abstract
Methods for quantitative fluorescent analysis reemphasize the dependence of scientific advances on technologic development. The techniques of fluorescent assay are versatile and relatively simple. Their sensitivity is comparable to that of radioactive assay methods, making possible the detection of trace amounts of many substances. This volume is a remarkably complete summary of a rapidly advancing field. A refreshing introduction reviews the relationship of physicists and biologists to the development of techniques for fluorescent assay and is followed by an essentially nonmathematical treatment of luminescence and fluorescence. Sections on instrumentation are detailed. The ultrasensitivity of fluorescence analysis leads to problems of contamination, self-absorption, and quantitative recovery similar to those with radioactive isotopes. These problems are carefully delineated by the author. Specific applications of fluorescent measurement constitute the bulk of the book. Of particular interest are methods for assay of antibiotics such as griseofulvin and the tetracyclines, catecholamines, plus fluorescent microscopy and
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Cited by
Studies on the Chemical Nature of Clostridial Ferredoxin
Lovenberg, W., Buchanan, B. B., & Rabinowitz, J. C. (1963) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51805-6 · Cited by 600
Riederer, P., & Wuketich, S. (1976) · Journal of Neural Transmission · doi:10.1007/bf01249445 · Cited by 577
Acid-Base Properties of Electronically Excited States of Organic Molecules
Ireland, J. F., & Wyatt, P. A. H. (1976) · Advances in Physical Organic Chemistry · doi:10.1016/s0065-3160(08)60331-7 · Cited by 572
PURINERGIC INNERVATION OF THE GUINEA‐PIG URINARY BLADDER
BURNSTOCK, G., COCKS, T., CROWE, R., & KASAKOV, L. (1978) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1978.tb07782.x · Cited by 317
Enzymic Studies on the Biosynthesis of Serotonin in Mammalian Brain
Ichiyama, A., Nakamura, S., Nishizuka, Y., & Hayaishi, O. (1970) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)77149-x · Cited by 311
Metadata
- Authors
- Crounse, R. G.
- Venue
- Archives of Dermatology
- Publisher
- American Medical Association (AMA)
- Published
- 1963-02-01
- Volume
- 87
- Issue
- 2
- Pages
- 283
- ISSN
- 0003-987X (ISSN-L)
- OpenAlex
- W2060412734
- Cited by
- 644
- References
- 0
- FWCI
- 112.04
- Type
- journal article
- Language
- en
- Open access
- closed
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