The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Abstract
The bacterial chromosome and bacterial plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage‐encoded recombination proteins efficiently recombine sequences with homologies as short as 35 to 50 bases. Recombineering allows DNA sequences to be inserted or deleted without regard to location of restriction sites. This unit first describes preparation of electrocompetent cells expressing the recombineering functions and their transformation with dsDNA or ssDNA. It then presents support protocols that describe several two‐step selection/counter‐selection methods of making genetic alterations without leaving any unwanted changes in the targeted DNA, and a method for retrieving onto a plasmid a genetic marker (cloning by retrieval) from the Escherichia coli chromosome or a co‐electroporated DNA fragment. Additional protocols describe methods to screen for unselected mutations, removal of the defective prophage from recombineering strains, and other useful techniques. Curr. Protoc. Mol. Biol. 106:1.16.1‐1.16.39. © 2014 by John Wiley & Sons, Inc.
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Cites
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429
Cited by
Isabella, V. M., Ha, B. N., Castillo, M. J., Lubkowicz, D. J., Rowe, S. E., Millet, Y. A., Anderson, C. L., Li, N., Fisher, A. B., West, K. A., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4222 · Cited by 605
Thomas, C. M., Hong, T., van Pijkeren, J. P., Hemarajata, P., Trinh, D. V., Hu, W., Britton, R. A., Kalkum, M., & Versalovic, J. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031951 · Cited by 491
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
Doman, J. L., Raguram, A., Newby, G. A., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0414-6 · Cited by 473
Alessa, O., Aiba, Y., Arbaah, M., Hidaka, Y., Watanabe, S., Miyanaga, K., Wannigama, D. L., & Cui, L. (2025) · Molecules · doi:10.3390/molecules30153132 · Cited by 21
Brown, A. N., Anderson, M. T., Smith, S. N., Bachman, M. A., & Mobley, H. L. T. (2023) · mBio · doi:10.1128/mbio.01448-23 · Cited by 14
Metadata
- Authors
- Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N., & Court, D. L.
- Venue
- Current Protocols in Molecular Biology
- Publisher
- Wiley
- Published
- 2014-04
- Volume
- 106
- Issue
- 1
- Pages
- 1.16.1-1.16.39
- ISSN
- 1934-3639 (ISSN-L)
- 1934-3647
- OpenAlex
- W1908889304
- Cited by
- 372
- References
- 1
- FWCI
- 18.43
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
