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Mycoplasma Contamination Of Cell Cultures
Abstract
Introduction Biology and Nomenclature of Mycoplasmas Characteristics of Mycoplasmas Taxonomy of Mycoplasmas Mycoplasma Contamination of Cell Cultures Incidence of Mycoplasma Contamination Most Common Contaminating Mycoplasma Species Sources of Mycoplasma Contamination Effects of Mycoplamsa Contamination Detection of Mycoplasma Contamination Various Detection Methods Most Reliable Detection Methods Elimination of Mycoplasma Contamination Various Elimination Methods Antibiotic Treatment as the Most Effective Method Working Protocols for Detection, Elimination and Prevention of Mycoplasma Contamination Routine Working Protocol for Detection Routine Working Protocol for Elimination Routine Working Protocol for Prevention Bibliography
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Mycoplasma infection of cell lines is usually not obvious.
“As mycoplasmainfected cell lines may show no obvious signs of infection -e.g.turbidity, reduced growth rate or viability, or visible cytopathic effect; infection is often insidious [3].”
From A highly sensitive internally-controlled real-time PCR assay for mycoplasma detection in cell cultures (2020) · cites this paper as [3]
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Cites
No references could be resolved yet.
Research works printed but not linked: 23
- Razin S., 1984, Bergey's Manual of Systematic Bacteriology, 740
- Razin S., 1991, The Prokaryotes, 1937
- Tully J.G., 1992, Encyclopedia of Microbiology, 181
- 10.1016/0167-7799(93)90089-R · DOI
- 10.1016/B978-012583805-4/50003-0 · DOI
- Razin S., Microbiol. Mol. Biol. Reviews
- 10.1128/jb.171.12.6455-6467.1989 · DOI
- 10.1007/978-1-4615-2478-6_12 · DOI
- Del Guidice R.A., 1984, Uses and Standardization of Vertebrate Cell Cultures—In Vitro Monograph No. 5, 104
- McGarrity G.J., 1985, Am. Soc. Microbiol. News, 51, 170
- 10.1038/339487a0 · DOI
- McGarrity G.J., 1992, Mycoplasmas—Molecular Biology and Pathogenesis, 445
- 10.1007/BF02633326 · DOI
- Uphoff C.C., 1992, Leukemia, 6, 335
- 10.1007/BF02615318 · DOI
- 10.1016/S0022-1759(12)80047-0 · DOI
- 10.1006/mcpr.1994.1071 · DOI · Paper page
- Fleckenstein E., 1994, Leukemia, 8, 1424
- 10.1101/gr.4.4.199 · DOI
- 10.1038/sj.leu.2402365 · DOI
- 10.1038/sj.leu.2402364 · DOI · Paper page
- 10.1007/BF02631456 · DOI
- Drexler H.G., 1995, DSMZ Catalogue of Human and Animal Cell Lines, 8
Cited by
Sorted by citations
Sung, J., & Hawkins, J. R. (2020) · Biologicals · doi:10.1016/j.biologicals.2019.12.007 · Cited by 22
Mycoplasma Contamination of Primary Cell Cultures
A. Leonovich, O. (2026) · Biochemistry · doi:10.5772/intechopen.1013412 · Cited by 1
Metadata
- Authors
- Drexler, H. G., & Uphoff, C. C.
- Affiliations
- Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DE)
- Venue
- Encyclopedia of Cell Technology
- Publisher
- Wiley
- Published
- 2000-01-25
- OpenAlex
- W2912793702
- Cited by
- 33
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2021: 1 · 2020: 2 · 2016: 1 · 2015: 3 · 2014: 6 · 2013: 1 · 2012: 1
- Type
- reference entry
- Language
- en
- Open access
- closed
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- Mycoplasma, mycoplasma contamination, cell culture contamination, mycoplasma detection, mycoplasma elimination, antibiotic therapy, mycoplasma taxonomy
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