Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression
Abstract
Mineralizing osteoblasts (MOBs) can release exosomes, although the functional significance remains unclear. In the present study, we demonstrate that exosomes derived from mineralizing pre-osteoblast MC3T3-E1 cells can promote bone marrow stromal cell (ST2) differentiation to osteoblasts. We reveal that MOB-derived exosomes significantly influence miRNA profiles in recipient ST2 cells, and these changes tend to activate the Wnt signaling pathway by inhibiting Axin1 expression and increasing β-catenin expression. We also suggest that MOB derived-exosomes partly induce the variation in miRNA expression in recipient ST2 cells by exosomal miRNA transfer. These findings suggest an exosome-mediated mode of cell-to-cell communication in the osteogenic microenvironment, and also indicate the potential of MOB exosomes in bone tissue engineering.
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The miRNA profile of MSC-Exos undergoes changes during osteogenic differentiation of MSCs as a result of gene expression reprogramming and organelle redistribution.
“Nevertheless, the miRNA profile of MSC-Exos undergoes changes during the osteogenic differentiation of MSCs as a result of gene expression reprogramming and organelle redistribution [28,29].”
From Hydrogel scaffold encapsulating MSC-Exos and ZIF-8 promotes bone regeneration via coordinating osteogenesis and immunomodulation (2025) · cites this paper as [29]
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References 18 · Cited by 292
- Authors
- Cui, Y., Luan, J., Li, H., Zhou, X., & Han, J.
- Affiliations
- Shandong First Medical University (CN)
- Corresponding author
- Han, Jinxiang
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2015-12-30
- Volume
- 590
- Issue
- 1
- Pages
- 185-192
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2239810497
- Cited by
- 292
- References
- 18
- Citations by year
- 2026: 13 · 2025: 11 · 2024: 35 · 2023: 27 · 2022: 33 · 2021: 52 · 2020: 39 · 2019: 29 · 2018: 26 · 2017: 19 · 2016: 8
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81371909)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- exosomes, mesenchymal stem cells, osteogenic differentiation, microRNA transfer, Wnt signaling, AXIN1, β-catenin, bone tissue engineering, MC3T3-E1 cells
- MeSH terms
- Animals, Calcification, Physiologic, Cell Communication, Cell Line, Expert Systems, Osteoblasts, Osteogenesis, Biomarkers, 3T3 Cells, Gene Expression Regulation, Developmental, Computational Biology, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, beta Catenin, Mice, Exosomes, Mesenchymal Stem Cells, Wnt Signaling Pathway, Axin Protein, Cellular Microenvironment
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