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Cited by 4

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TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors

Hawley, Robert G., Hawley, Teresa S., Cantor, Alan B.·2008·Current Protocols in Stem Cell Biology
DOI 10.1002/9780470151808.sc01f07s7PMC PMC2633409
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Abstract

The ability to generate genetically engineered cell lines is of great experimental value. They provide a renewable source of material that may be suitable for biochemical analyses, chromatin immunoprecipitation assays, structure‐function studies, gene function assignment, and transcription factor target gene identification. This unit describes protocols for TLX1 (HOX11)‐mediated immortalization of murine hematopoietic progenitors derived from in vitro differentiated murine embryonic stem cells, or from primary mouse fetal liver or bone marrow. A wide variety of hematopoietic cell types have been immortalized using these procedures including erythroid, megakaryocytic, monocytic, myelocytic, and multipotential cell types. These lines are typically cytokine dependent for their survival and growth. Curr. Protoc. Stem Cell Biol. 7:1F.7.1‐1F.7.19. © 2008 by John Wiley & Sons, Inc.

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Cited by

Metadata

Authors
Hawley, R. G., Hawley, T. S., & Cantor, A. B.
Affiliations
  • The George Washington University Medical Center Washington D.C.
  • Flow Cytometry Core Facility, The George Washington University Medical Center Washington D.C.
  • Department of Pediatric Hematology‐Oncology, Childrens's Hospital Boston Boston Massachusetts
Venue
Current Protocols in Stem Cell Biology
Publisher
Wiley
Published
2008-12
Volume
7
Issue
1
ISSN
  • 1941-7322
  • 1938-8969
DOI
10.1002/9780470151808.sc01f07s7
PMC
PMC2633409
Cited by
4
References
2
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)

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