The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Target Identification Using Drug Affinity Responsive Target Stability (DARTS)
Open accessAbstract
Drug affinity responsive target stability (DARTS) is a general methodology for identifying and studying protein‐ligand interactions. The technique is based on the principle that when a small molecule compound binds to a protein, the interaction stabilizes the target protein's structure such that it becomes resistant to proteases. DARTS is particularly useful for the initial identification of the protein targets of small molecules, but can also be used to validate potential protein‐ligand interactions predicted or identified by other means and to estimate the affinity of interactions. The approach is simple and advantageous because it can be performed using crude cell lysates and other complex protein mixtures (without requiring purified proteins), and it uses native, unmodified small molecules. The protocols in this unit describe the general approach for performing DARTS experiments, which can be easily modified and scaled to fit project‐specific criteria. Curr. Protoc. Chem. Biol. 3:163‐180 © 2011 by John Wiley & Sons, Inc.
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Cites
Universal sample preparation method for proteome analysis
Wiśniewski, J. R., Zougman, A., Nagaraj, N., & Mann, M. (2009) · Nature Methods · doi:10.1038/nmeth.1322 · Cited by 8,352
Global analysis of protein expression in yeast
Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., & Weissman, J. S. (2003) · Nature · doi:10.1038/nature02046 · Cited by 3,750
Global Analysis of Protein Activities Using Proteome Chips
Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., et al. (2001) · Science · doi:10.1126/science.1062191 · Cited by 1,884
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Chin, R. M., Fu, X., Pai, M. Y., Vergnes, L., Hwang, H., Deng, G., Diep, S., Lomenick, B., Meli, V. S., Monsalve, G. C., et al. (2014) · Nature · doi:10.1038/nature13264 · Cited by 608
Self-citationZhu, Y., Ouyang, Z., Du, H., Wang, M., Wang, J., Sun, H., Kong, L., Xu, Q., Ma, H., & Sun, Y. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.08.022 · Cited by 414
Drug Affinity Responsive Target Stability (DARTS) for Small-Molecule Target Identification
Pai, M. Y., Lomenick, B., Hwang, H., Schiestl, R., McBride, W., Loo, J. A., & Huang, J. (2015) · Methods in Molecular Biology · doi:10.1007/978-1-4939-2269-7_22 · Cited by 354
Self-citationUtilizing graph machine learning within drug discovery and development
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Metadata
- Authors
- Lomenick, B., Jung, G., Wohlschlegel, J. A., & Huang, J.
- Venue
- Current Protocols in Chemical Biology
- Publisher
- Wiley
- Published
- 2011-12
- Volume
- 3
- Issue
- 4
- Pages
- 163-180
- ISSN
- 2160-4762 (ISSN-L)
- OpenAlex
- W2172993862
- Cited by
- 238
- References
- 3
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- green
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