Hydrogel Design of Experiments Methodology to Optimize Hydrogel for iPSC‐NPC Culture
Open accessAbstract
Bioactive signals can be incorporated in hydrogels to direct encapsulated cell behavior. Design of experiments methodology methodically varies the signals systematically to determine the individual and combinatorial effects of each factor on cell activity. Using this approach enables the optimization of three ligands concentrations (RGD, YIGSR, IKVAV) for the survival and differentiation of neural progenitor cells.
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Hyaluronic acid and mixtures of hyaluronic acid and chitosan have been extensively used in the growth and construction of neural organoids.
“Similarly, hyaluronic acid and mixtures of hyaluronic acid and chitosan have been extensively used in the growth and construction of neural organoids111–116.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [115]
The concentration of laminin-derived IKVAV, with 300 μM being the optimal concentration, was critical to neural organoid survival; however, this concentration did not enhance neuronal differentiation.
“Lam and colleagues found that the concentration of laminin-derived IKVAV, with 300 μM being the optimal concentration, was critical to neural organoid survival; however, this concentration did not enhance neuronal differentiation115.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [115]
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References 26 · Cited by 119
Research works printed but not linked: 3
- [12]Hubbell JA Lutolf MP Lauer-Fields JL Schmoekel HG Metters AT Weber FE Fields GB P Natl Acad Sci USA 2003 100 5413 PMC154359 12686696
- [13]Adeloew C Segura T Hubbell JA Frey P Biomaterials 2008 29 314 17953986
- [17]Discher DE Sweeney L Sen S Engler A Biophysical Journal 2007 32a
- Authors
- Lam, J., Carmichael, S. T., Lowry, W. E., & Segura, T.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Segura, Tatiana
- Venue
- Advanced Healthcare Materials
- Publisher
- Wiley
- Published
- 2014-11-05
- Volume
- 4
- Issue
- 4
- Pages
- 534-539
- ISSN
- 2192-2640 (ISSN-L)
- 2192-2659
- PMC
- PMC4384641
- OpenAlex
- W2092751347
- Cited by
- 119
- References
- 26
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 9 · 2023: 9 · 2022: 9 · 2021: 14 · 2020: 10 · 2019: 14 · 2018: 12 · 2017: 8 · 2016: 15 · 2015: 3
- FWCI
- 3.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Healthcare Materials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL110592)
- National Institute of General Medical Sciences (P01GM099134, T32GM067555)
- National Institute of Neurological Disorders and Stroke (R01NS079691)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- hydrogels, experimental design, RGD peptide, YIGSR peptide, IKVAV peptide, cell encapsulation, neural progenitor cell differentiation, cell survival
- MeSH terms
- Amino Acid Sequence, Cell Adhesion, Humans, Hyaluronic Acid, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Peptides, Cell Culture Techniques, Hydrogel, Polyethylene Glycol Dimethacrylate, Induced Pluripotent Stem Cells, Neural Stem Cells
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