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A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae
Abstract
A method for the rapid detection of cyanobacteria (blue-green algae) and their differentiation from eukaryotic algae in natural phytoplankton assemblages is presented. Fluorescence emission of photosynthetic pigments at 670 nm was measured using a microplate fluorescence reader when excited at two different wavelengths – 485 nm and 590 nm. The ratio of fluorescence excited at these wavelengths (590 nm/485 nm) was proportional to the ratio of cyanobacteria and eukaryotic algae, which was determined by the in situ spectrofluorometer for the phytoplankton quantification. The fluorescence intensity was equal to the total chlorophyll-a content. These two fluorescence values can provide the first warning on a development of potentially toxic cyanobacteria in water.
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“Other reports show the potential of this technique to distinguish several chlorophylls (a, b, and c) and their degradation products (pheophytin a and b, and pheoporphyrin c in acetone: water model‐solutions (Moberg et al., 2001) and the possibility to estimate algal blooms (Gregor and Maršálek, 2005; Ziegmann et al., 2010).”
From Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry (2022) · cites this paper as Gregor and Maršálek, 2005
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References 14 · Cited by 64
Research works printed but not linked: 18
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Metadata
- Authors
- Gregor, J., & Maršálek, B.
- Affiliations
- Czech Academy of Sciences (CZ)
- Corresponding author
- Gregor, Jakub
- Venue
- Acta hydrochimica et hydrobiologica
- Publisher
- Wiley
- Published
- 2005-06
- Volume
- 33
- Issue
- 2
- Pages
- 142-148
- ISSN
- 0323-4320 (ISSN-L)
- 1521-401X
- OpenAlex
- W2084070218
- Cited by
- 64
- References
- 14
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 2 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 4 · 2013: 8 · 2012: 3
- FWCI
- 1.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta hydrochimica et hydrobiologica (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Environmental Science › Waste Management and Disposal › Water Quality Monitoring and Analysis
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
- Keywords
- cyanobacteria, eukaryotic algae, freshwater phytoplankton, fluorescence spectroscopy, chlorophyll a, photosynthetic pigments, microplate reader, cyanotoxins
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- CoChat. (2026). Citation check: A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae. https://app.cochat.ai/papers/10.1002/aheh.200400558
- The paper
- Gregor, J., & Maršálek, B. (2005). A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae. Acta hydrochimica et hydrobiologica. https://doi.org/10.1002/aheh.200400558
