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Effect of heparin on platelet aggregation
Abstract
The effect of heparin on platelet aggregation was systematically examined on platelets in plasma (PRP), as well as on gel‐filtered, washed, and formaldehyde‐fixed platelets. Results indicate that, although heparin causes a mild potentiation of platelet aggregation in the PRP systems, a significant inhibitory activity is observed when heparin is added to isolated platelets. This inhibitory activity appears to be specific and not related to the impurities in the heparin preparations, as heparinase, as well as protamine, effectively neutralizes the heparin‐mediated inhibitory activity on platelet aggregation. Although heparin‐mediated inhibitory activity can be demonstrated in the presence of a number of different agonists (ADP, arachidonic acid, thrombin, Ionophore A23187, epinephrine, and ristocetin), the most pronounced inhibition is seen in the presence of ristocetin. Further studies show that heparin enhances thrombox‐ane generation in isolated platelets. Platelets pretreated with heparin, however, fail to respond to preformed thromboxane. These findings suggest that, in addition to the potentiation of thromboxane production in platelets, heparin may also attribute some change(s) to the platelet(s)/platelet membrane, which interferes with their ability to respond to the agonists of platelet aggregation. This antiaggregatory activity of heparin was found to be inhibited by a factor(s) present in plasma but not in serum.
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“Blood differential cell counts were carried out on fresh whole blood collected in EDTA rather than heparin tubes to avoid heparin-induced aggregation of platelets77, using a Coulter Ac•T diff2™ Hematology Analyzer (Beckman-Coulter Corp., Miami, FL).”
From The effect of smoking on chronic inflammation, immune function and blood cell composition (2020) · cites this paper as [77]
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Research works printed but not linked: 33
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Cited by
The effect of smoking on chronic inflammation, immune function and blood cell composition
Elisia, I., Lam, V., Cho, B., Hay, M., Li, M. Y., Yeung, M., Bu, L., Jia, W., Norton, N., Lam, S., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-76556-7 · Cited by 263
Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges
Elisia, I., Lam, V., Hofs, E., Li, M. Y., Hay, M., Cho, B., Brooks-Wilson, A., Rosin, M., Bu, L., Jia, W., et al. (2017) · PLOS ONE · doi:10.1371/journal.pone.0188881 · Cited by 34
Metadata
- Authors
- Saba, H. I., Saba, S. R., & Morelli, G. A.
- Venue
- American Journal of Hematology
- Publisher
- Wiley
- Published
- 1984-11
- Volume
- 17
- Issue
- 3
- Pages
- 295-306
- ISSN
- 0361-8609
- 1096-8652
- Cited by
- 58
- References
- 0
- Language
- en
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