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A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes
Abstract
On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.
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Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
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Metadata
- Authors
- Vogelsang, J., Kasper, R., Steinhauer, C., Person, B., Heilemann, M., Sauer, M., & Tinnefeld, P.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2008-07-07
- Volume
- 47
- Issue
- 29
- Pages
- 5465-5469
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2100483890
- Cited by
- 618
- References
- 1
- FWCI
- 22.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vogelsang, J., Kasper, R., Steinhauer, C., Person, B., Heilemann, M., Sauer, M., & Tinnefeld, P. (2008). A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.200801518
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