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Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF4 Nanocrystals
Abstract
Background elimination and improved sensitivity were achieved by time-resolved (TR) detection with a FRET biosensor for traces of biomolecules such as avidin at concentrations down to 4.8 nM. As shown in the picture, UV excitation of biotinylated NaYF4:Ce/Tb nanocrystals triggers energy transfer to fluorescein isothiocyanate (FITC), whose long-lived emission due to FRET can be distinguished from the short-lived background from direct excitation.
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Cited by
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Self-citationRare-Earth Doping in Nanostructured Inorganic Materials
Zheng, B., Fan, J., Chen, B., Qin, X., Wang, J., Wang, F., Deng, R., & Liu, X. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00644 · Cited by 1,204
Zheng, W., Huang, P., Tu, D., Ma, E., Zhu, H., & Chen, X. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00178h · Cited by 894
Self-citationDoped quantum dots for chemo/biosensing and bioimaging
Wu, P., & Yan, X.-P. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60017c · Cited by 667
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Metadata
- Authors
- Tu, D., Liu, L., Ju, Q., Liu, Y., Zhu, H., Li, R., & Chen, X.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2011-05-24
- Volume
- 50
- Issue
- 28
- Pages
- 6306-6310
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2144308433
- Cited by
- 330
- References
- 0
- FWCI
- 22.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tu, D., Liu, L., Ju, Q., Liu, Y., Zhu, H., Li, R., & Chen, X. (2011). Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF4 Nanocrystals. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201100303
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