Protection and Deprotection of DNA—High‐Temperature Stability of Nucleic Acid Barcodes for Polymer Labeling

DNA-based files are encapsulated physically within discrete, impervious silica capsules, which are subsequently surface-labeled with unique single-stranded DNA barcodes that offer Boolean logic based selection on the entire data pool via simple hybridization.

“To realize this file system, we first encapsulate DNA-based files physically within discrete, impervious silica capsules9,23,24, which we subsequently surface-label with unique single-stranded DNA barcodes that offer Boolean logic based selection on the entire data pool via simple hybridization.”

Because physical encapsulation separates file sequences from external barcodes, the file system offers long-term environmental protection of encoded file sequences via silica encapsulation for permanent archival storage, where external barcodes may be renewed periodically, further protected with secondary encapsulation, or data pools may be stored using methods implemented in PCR-based random

“Because physical encapsulation separates file sequences from external barcodes that are used to describe the encapsulated information, our file system offers long-term environmental protection of encoded file sequences via silica encapsulation for permanent archival storage9,23,24, where external barcodes may be renewed periodically, further protected with secondary encapsulation, or data pools”