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Cited by 158

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Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super‐Resolution RESOLFT Microscopy

Open access
Roubinet, Benoît, Bossi, Mariano L., Alt, Philipp, Leutenegger, Marcel, Shojaei, Heydar, Schnorrenberg, Sebastian, Nizamov, Shamil, Irie, Masahiro, Belov, Vladimir N., Hell, Stefan W.·2016·Angewandte Chemie International Edition

Abstract

Reversibly photoswitchable 1,2‐bis(2‐ethyl‐6‐phenyl‐1‐benzothiophene‐1,1‐dioxide‐3‐yl)perfluorocyclopentenes (EBT) having fluorescent “closed” forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology‐related super‐resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.

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Metadata

Authors
Roubinet, B., Bossi, M. L., Alt, P., Leutenegger, M., Shojaei, H., Schnorrenberg, S., Nizamov, S., Irie, M., Belov, V. N., & Hell, S. W.
Venue
Angewandte Chemie International Edition
Publisher
Wiley
Published
2016-10-21
Volume
55
Issue
49
Pages
15429-15433
ISSN
  • 1433-7851 (ISSN-L)
  • 1521-3773
OpenAlex
W2533608121
Cited by
158
References
1
FWCI
8.73
Type
journal article
Language
en
Open access
hybrid

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Roubinet, B., Bossi, M. L., Alt, P., Leutenegger, M., Shojaei, H., Schnorrenberg, S., Nizamov, S., Irie, M., Belov, V. N., & Hell, S. W. (2016). Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super‐Resolution RESOLFT Microscopy. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201607940

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.