Involvement of αvβ5 integrin–mediated activation of latent transforming growth factor β1 in autocrine transforming growth factor β signaling in systemic sclerosis fibroblasts

Asano, Yoshihde; Ihn, Hironobu; Yamane, Kenichi; Jinnin, Masatoshi; Mimura, Yoshihiro; Tamaki, Kunihiko

doi:10.1002/art.21246

Involvement of αvβ5 integrin–mediated activation of latent transforming growth factor β1 in autocrine transforming growth factor β signaling in systemic sclerosis fibroblasts

Objective To confirm the involvement of αvβ5 in the self‐activation system in systemic sclerosis (SSc) fibroblasts. Methods Levels of αvβ5 expression were analyzed by immunoprecipitation. The promoter activity of the human α2(I) collagen gene was determined by transient transfection assay. Phosph…