Cold stress improves the production of artemisinin depending on the increase in endogenous jasmonate
Abstract
Previous publications reported that the artemisinin level was increased in Artemisia annua following a night‐frost period. However, the molecular mechanism was not clear. In this study, we found that exogenous jasmonate (JA) effectively enhanced the freezing tolerance of A. annua. The JA biosynthetic genes (LOX1, LOX2, allene oxide cyclase [AOC], and jasmonate resistant 1 [JAR1]) were induced by cold stress, leading to an increase in endogenous JA in cold‐treated A. annua. Increased endogenous JA enhanced the expression of three JA‐responsive transcription factors, ethylene response factor 1, ethylene response factor 2, and octadecanoid‐responsive AP2/ERF, all of which were reported to transcriptionally activate the expression of artemisinin biosynthetic genes, such as amorpha‐4,11‐diene synthase (ADS), CYP71AV1, DBR2, and aldehyde dehydrogenase 1 (ALDH1). Furthermore, the expression levels of the four artemisinin biosynthetic genes were also significantly increased under cold stress. Consequently, the levels of artemisinin and related secondary metabolites, such as dihydroartemisinic acid, artemisinin B, and artemisinic acid, were increased in A. annua under cold stress. Our study points to a molecular mechanism in which the production of artemisinin is regulated by cold stress in A. annua.
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Low temperature treatment of Artemisia plants at 4 °C for 12 to 48 h increased artemisinin content compared to levels at 0 h.
“Liu et al. (2017) reported that low temperature treatment of Artemisia plants at 4 °C for 12 to 48 h increased artemisinin content compared to levels at 0 h.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Liu et al. 2017
These transcription factors act as transcriptional activators of artemisinin biosynthetic genes including ADS, DBR2, CYP71AV1, and ALDH1.
“These transcription factors act as transcriptional activators of artemisinin biosynthetic genes including ADS , DBR2 , CYP71AV1 , and ALDH1 (Liu et al. 2017).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Liu et al. 2017
The increase in endogenous JA led to enhanced expression of JA-responsive transcription factors octadecanoid-responsive AP2/ERF, ethylene response factor 1, and ethylene response factor 2.
“The increase in endogenous JA in turn led to enhanced expression of JA-responsive transcription factors, namely octadecanoid -responsive AP2/ERF, ethylene response factor 1, and ethylene response factor 2.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Liu et al. 2017
The low-temperature increase in artemisinin appeared to result from an increase in endogenous JA due to activation of JA biosynthetic genes LOX1, LOX2, JAR1 and AOC.
“This increase appeared to be the result of an increase in endogenous JA, due to activation of JA biosynthetic genes like LOX1 , LOX2 , JAR1 and AOC responding to cold stress.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Liu et al. 2017
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References 45 · Cited by 68
- Authors
- Liu, W., Wang, H., Chen, Y., Zhu, S., Chen, M., Lan, X., Chen, G., & Liao, Z.
- Affiliations
- Southwest University (CN)
- Tibet University (CN)
- Key Laboratory of Biorheological Science and Technology (Ministry of Education) College of Bioengineering Chongqing University Chongqing People's Republic of China
- Corresponding author
- Liao, Zhihua
- Venue
- Biotechnology and Applied Biochemistry
- Publisher
- Wiley
- Published
- 2016-10-10
- Volume
- 64
- Issue
- 3
- Pages
- 305-314
- ISSN
- 0885-4513 (ISSN-L)
- 1470-8744
- OpenAlex
- W2301861642
- Cited by
- 68
- References
- 45
- Citations by year
- 2026: 3 · 2025: 13 · 2024: 13 · 2023: 7 · 2022: 10 · 2021: 6 · 2020: 11 · 2019: 2 · 2018: 2 · 2016: 1
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Applied Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31070266, 31200223)
- Program for New Century Excellent Talents in University (NCET‐12‐0930)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- artemisinin biosynthesis, cold stress, jasmonates, Artemisia annua, freezing tolerance, LOX, allene oxide cyclase, JAR1, ethylene response factor, AP2/ERF transcription factors, secondary metabolites, dihydroartemisinic acid, artemisinic acid
- MeSH terms
- Cyclopentanes, Lactones, Plant Proteins, Gene Expression Regulation, Plant, Artemisia annua, Artemisinins, Oxylipins, Cold-Shock Response
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