Identification of a malonyl CoA‐acyl carrier protein transacylase and its regulatory role in fatty acid biosynthesis in oleaginous microalga Nannochloropsis oceanica
Abstract
Oleaginous microalgae hold great promises for biofuel production. However, commercialization of microalgal biofuels remains impracticable due to the lack of suitable industrial strains with high growth rate and lipid productivity. Engineering of metabolic pathways is a potential strategy for the improvement of microalgal strains for the production of lipids and also value‐added products in microalgae. Malonyl CoA‐acyl carrier protein transacylase (MCAT) has been reported to be involved in fatty acid biosynthesis. Here, we identified a putative MCAT in the oleaginous marine microalga Nannochloropsis oceanica. NoMCAT overexpressing N. oceanica showed a higher growth rate and photosynthetic efficiency. The neutral lipid content of engineered lines showed a significant increase by up to 31% compared to wild type. Gas chromatography–mass spectrometry analysis revealed that NoMCAT overexpression significantly altered the fatty acid composition. The composition of eicosapentaenoic acid (C20:5), which is a polyunsaturated fatty acid necessary for animal nutrition, increased by 8%. These results demonstrate the role of MCAT in enhancing fatty acid biosynthesis and growth in microalgae, and also provide an insight into metabolic engineering of microalgae with high industrial potential.
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Overexpressing Malonyl CoA-acylcarrier protein transacylase gene and glycerol-3-phosphate acyltransferases gene promoted neutral lipid accumulation in microalgae.
“Overexpressing Malonyl CoA-acylcarrier protein transacylase gene and glycerol-3-phosphate acyltransferases gene promoted neutral lipid accumulation in microalgae, too [186, 187].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [186]
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References 21 · Cited by 111
- Authors
- Chen, J., Liu, W., Hu, D., Wang, X., Balamurugan, S., Alimujiang, A., Yang, W., Liu, J., & Li, H.
- Affiliations
- Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes College of Life Science Jinan University Guangzhou People's Republic of China
- Jinan University (CN)
- Venue
- Biotechnology and Applied Biochemistry
- Publisher
- Wiley
- Published
- 2017-04-18
- Volume
- 64
- Issue
- 5
- Pages
- 620-626
- ISSN
- 0885-4513 (ISSN-L)
- 1470-8744
- OpenAlex
- W2509697365
- Cited by
- 111
- References
- 21
- Citations by year
- 2026: 11 · 2025: 19 · 2024: 12 · 2023: 9 · 2022: 13 · 2021: 12 · 2020: 13 · 2019: 10 · 2018: 6 · 2017: 6
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Applied Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (41576132)
- Natural Science Foundation of Guangdong Province (2014A030308010)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Nannochloropsis oceanica, oleaginous microalgae, fatty acid synthesis, microalgal lipid production, eicosapentaenoic acid, fatty acid composition, microalgae growth rate, photosynthetic efficiency, metabolic engineering, biofuel production
- MeSH terms
- Fatty Acids, Acyl-Carrier Protein S-Malonyltransferase, Biofuels, Stramenopiles, Microalgae
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