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Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications
Abstract
Protein fusion tags are indispensible tools used to improve recombinant protein expression yields, enable protein purification, and accelerate the characterization of protein structure and function. Solubility‐enhancing tags, genetically engineered epitopes, and recombinant endoproteases have resulted in a versatile array of combinatorial elements that facilitate protein detection and purification in microbial hosts. In this comprehensive review, we evaluate the most frequently used solubility‐enhancing and affinity tags. Furthermore, we provide summaries of well‐characterized purification strategies that have been used to increase product yields and have widespread application in many areas of biotechnology including drug discovery, therapeutics, and pharmacology. This review serves as an excellent literature reference for those working on protein fusion tags.
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Global analysis of protein expression in yeast
Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., & Weissman, J. S. (2003) · Nature · doi:10.1038/nature02046 · Cited by 3,750
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Cho, N. H., Cheveralls, K. C., Brunner, A.-D., Kim, K., Michaelis, A. C., Raghavan, P., Kobayashi, H., Savy, L., Li, J. Y., Canaj, H., et al. (2022) · Science · doi:10.1126/science.abi6983 · Cited by 567
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Costa, S., Almeida, A., Castro, A., & Domingues, L. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00063 · Cited by 450
Crozet, P., Navarro, F. J., Willmund, F., Mehrshahi, P., Bakowski, K., Lauersen, K. J., Pérez-Pérez, M.-E., Auroy, P., Gorchs Rovira, A., Sauret-Gueto, S., et al. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00251 · Cited by 297
Metadata
- Authors
- Young, C. L., Britton, Z. T., & Robinson, A. S.
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2012-01-10
- Volume
- 7
- Issue
- 5
- Pages
- 620-634
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2171377206
- Cited by
- 505
- References
- 1
- FWCI
- 12.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- Young, C. L., Britton, Z. T., & Robinson, A. S. (2012). Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications. Biotechnology Journal. https://doi.org/10.1002/biot.201100155
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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