CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives
Abstract
Chinese hamster ovary (CHO) cells are the most widely used production host for therapeutic proteins. With the recent emergence of CHO genome sequences, CHO cell line engineering has taken on a new aspect through targeted genome editing. The bacterial clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system enables rapid, easy and efficient engineering of mammalian genomes. It has a wide range of applications from modification of individual genes to genome-wide screening or regulation of genes. Facile genome editing using CRISPR/Cas9 empowers researchers in the CHO community to elucidate the mechanistic basis behind high level production of proteins and product quality attributes of interest. In this review, we describe the basis of CRISPR/Cas9-mediated genome editing and its application for development of next generation CHO cell factories while highlighting both future perspectives and challenges. As one of the main drivers for the CHO systems biology era, genome engineering with CRISPR/Cas9 will pave the way for rational design of CHO cell factories.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The availability of genomic sequences has enabled CHO cell engineering with unprecedented precision using engineered nucleases including ZFNs, TALENs and CRISPR.
“The availability of genomic sequences has enabled CHO cell engineering with unprecedented precision using engineered nucleases, including zinc finger nucleases (ZFNs) and transcription activator like nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR) system [71–73,75,76].”
From Glycoengineering Chinese hamster ovary cells: a short history (2021) · cites this paper as [75]
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 123 · Cited by 117
- Authors
- Lee, J. S., Grav, L. M., Lewis, N. E., & Faustrup Kildegaard, H.
- Affiliations
- Novo Nordisk Foundation (DK)
- University of California San Diego (US)
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2015-06-09
- Volume
- 10
- Issue
- 7
- Pages
- 979-994
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2117763379
- Cited by
- 117
- References
- 123
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 7 · 2022: 10 · 2021: 21 · 2020: 7 · 2019: 13 · 2018: 14 · 2017: 17 · 2016: 8 · 2015: 5
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Journal (publishedVersion)
- PubMed (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Funders
- The Novo Nordisk Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- CRISPR/Cas9, genome editing, CHO cells, therapeutic protein production, cell line engineering, genome-wide screen, gene expression regulation
- MeSH terms
- Animals, Cricetulus, Cricetinae, Humans, CHO Cells, Genome, Cell Engineering, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
