Glycans‐by‐design: Engineering bacteria for the biosynthesis of complex glycans and glycoconjugates
Abstract
There is an urgent need for new tools that enable better understanding of the structure, recognition, metabolism, and biosynthesis of glycans as well as the production of biologically important glycans and glycoconjugates. With the discovery of glycoprotein synthesis in bacteria and functional transfer of glycosylation pathways between species, Escherichia coli cells have become a tractable host for both understanding glycosylation and the underlying glycan code of living cells as well as for expressing glycoprotein therapeutics and vaccines. Here, we review recent efforts to harness natural biological pathways and engineer synthetic designer pathways in bacteria for making complex glycans and conjugating these to lipids and proteins. The result of these efforts has been a veritable transformation of bacteria into living factories for scalable, bottom‐up production of complex glycoconjugates by design. Biotechnol. Bioeng. 2013; 110: 1550–1564. © 2013 Wiley Periodicals, Inc.
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The absence of necessary activated sugar substrate UDP-GalNAc in laboratory E. coli strains requires co-expression of an additional UDP-GlcNAc 4-epimerase to generate UDP-GalNAc from UDP-GlcNAc.
“The absence of the necessary activated sugar substrate (UDP-GalNAc) in laboratory E. coli strains requires the co-expression of an additional UDP-GlcNAc 4-epimerase to generate UDP-GalNAc from UDP-GlcNAc [9, 20].”
From High level in vivo mucin-type glycosylation in Escherichia coli (2018) · cites this paper as [20]
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Roles of N-Linked Glycans in the Endoplasmic Reticulum
Helenius, A., & Aebi, M. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073752 · Cited by 2,037 · see citing works
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Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives
Baeshen, M. N., Al-Hejin, A. M., Bora, R. S., Ahmed, M. M. M., Ramadan, H. A. I., Saini, K. S., Baeshen, N. A., & Redwan, E. M. (2015) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1412.12079 · Cited by 330 · see citing works
High level in vivo mucin-type glycosylation in Escherichia coli
Mueller, P., Gauttam, R., Raab, N., Handrick, R., Wahl, C., Leptihn, S., Zorn, M., Kussmaul, M., Scheffold, M., Eikmanns, B., et al. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-1013-9 · Cited by 22 · see citing works
- Authors
- Merritt, J. H., Ollis, A. A., Fisher, A. C., & DeLisa, M. P.
- Affiliations
- Glycobia (United States) (US)
- Cornell University (US)
- Corresponding author
- DeLisa, Matthew P.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2013-03-26
- Volume
- 110
- Issue
- 6
- Pages
- 1550-1564
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2000823773
- Cited by
- 51
- References
- 1
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 8 · 2020: 5 · 2019: 3 · 2018: 7 · 2017: 3 · 2016: 4 · 2015: 5 · 2014: 4
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R44GM088905)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- glycans, glycoconjugates, glycosylation, Escherichia coli, lipid conjugation, protein conjugation
- MeSH terms
- Bacteria, Escherichia coli, Glycoconjugates, Glycosylation, Polysaccharides, Metabolic Engineering
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