Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time

Feichtinger, Julia, Hernández, Inmaculada, Fischer, Christoph, Hanscho, Michael, Auer, Norbert, Hackl, Matthias, Jadhav, Vaibhav, Baumann, Martina +11 more·2016·Biotechnology and Bioengineering
DOI 10.1002/bit.25990

Cited by (148)

Items 101–148 of 148

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  2. Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results
    Shuichi Kimura, Takeshi Ōmasa · 2018 · Cytotechnology
  3. Recent developments in miRNA based recombinant protein expression in CHO
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  4. The Epigenetic Component in Cancer Evolution
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  5. Stable overexpression of native and artificial miRNAs for the production of differentially fucosylated antibodies in CHO cells
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  6. Advancing Biologics Development Programs with Legacy Cell Lines: Advantages and Limitations of Genetic Testing for Addressing Clonality Concerns Prior to Availability of Late Stage Process and Product Consistency Data
    Paul Y K Wu, Taymar E. Hartman, Louise Almond, Jennitte L. Stevens, John Thrift, Juhi Ojha, Christina S. Alves, David Shaw, Michael W. Laird, Robyn Emmins et al. · 2019 · PDA Journal of Pharmaceutical Science and Technology
  7. Chromatin Accessibility Plays an Important Epigenetic Role on Antibody Expression From CMV Promoter and DNA Elements Flanking the CHO TI Host Landing‐Pad
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  8. Selective Recruitment of a Synthetic Histone Acetyltransferase Can Boost CHO Cell Productivity
    Sienna P. Butterfield, Rebecca E. Sizer, Fay L. Saunders, Robert J. White · 2024 · Biotechnology Journal
  9. Identifying model error in metabolic flux analysis – a generalized least squares approach
    Stanislav Sokolenko, Marco Quattrociocchi, Marc Gordon Aucoin · 2016 · BMC Systems Biology
  10. Improved recombinant protein production by regulation of transcription and protein transport in Chinese hamster ovary cells
    Kaiming Chen, Dong Li, Hongwen Li, Bing Li, Ruiqin Wang, Lingxiao Jiang, Lei Huang, Xiaoqing Xu, Jie Li, Fei Teng et al. · 2019 · Biotechnology Letters
  11. An Omic’s Data-Driven Approach Towards Engineering Mammalian Cell Factories and Bioprocesses for Biopharmaceutical Production
    Mauro Torres, Veronica Ortuzar, Alan James Dickson, Hirra Hussain · 2021 · Cell engineering
  12. Identification of Loci with High Transgene Expression in CHO Cells
    Ipek Tasan, Ittai E. Eres, Christopher Wei, Eric Edward Bryant, Emil Brisan, René Hubert · 2025 · ACS Synthetic Biology
  13. Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank
    Guanghua Li, Jennifer M. Pollard, Ren Liu, Richard C. Stevens, Jorge Quiroz, Michael C. Nelson, Matthew Manahan, Nicholas Murgolo, Robin S. Ehrick, Eric J. Wallenstein et al. · 2021 · Biotechnology Progress
  14. Artificial induction of chromosome aneuploidy in CHO cells alters their function as host cells
    Noriko Yamano, Yuto Nakanishi, Wataru Tanaka, Yuanshan Lai, Masahiro Yamazaki, Laura Zenner, Hirofumi Hata, Takeshi Ōmasa · 2022 · Biotechnology and Bioengineering
  15. Use of tRNA gene barriers improves stability of transgene expression in CHO cells
    Rebecca E. Sizer, Richard M. Ingram, Caroline Swan, Emma K. Biggs, Leon P. Pybus, Robert J. White · 2024 · Biotechnology Journal
  16. Mathematical Modelling of Cell Culture Processes
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  17. Comprehensive Mapping of Functional Enhancers in Chinese Hamster Ovary Cells
    María Santos, Yusuf B. Johari, Laura Biggins, Natalina Elliott, Stefan Schoenfelder, Mounika Boddireddy, Daniel K. Fabian, Michael Anbar, Peter M. O’Callaghan, Peter J. Rugg‐Gunn · 2025 · Biotechnology and Bioengineering
  18. The Local South American Chicken Populations Are a Melting-Pot of Genomic Diversity
    Agusto Luzuriaga-Neira, Lucía Pérez-Pardal, Sean M. O’Rourke, Gustavo Villacís-Rivas, Fredy Cueva Castillo, Galo Vinicio Escudero Sanchez, Juan Carlos Aguirre-Pabón, Amarilis Ulloa-Núñez, M. Quezada, Marcelo Vallinoto et al. · 2019 · Frontiers in Genetics
  19. Stepwise activation of gene copies results in higher final titers of subclones compared to immediate integration of the full set of active copies
    Víctor Jiménez Lancho, Peter Eisenhut, Gerald Klanert, Daniel Ivansson, Andreas Jonsson, Ann Lövgren, Nicole Borth · 2025 · New Biotechnology
  20. Genome Variation, the Epigenome and Cellular Phenotypes
    Martina Baumann, Gerald Klanert, Sabine Vcelar, Marcus Weinguny, Nicolas Marx, Nicole Borth · 2019
  21. Enhancing Antibody-Specific Productivity: Unraveling the Impact of XBP1s Overexpression and Glutamine Availability in SP2/0 Cells
    Priscilla González-Pereira, Ryan K. Trinh, Alex Vasuthasawat, Angelo Bartsch‐Jiménez, Constanza Núñez-Soto, Claudia Altamirano · 2024 · Bioengineering
  22. The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
    Hussain Dahodwala, Susan T. Sharfstein · 2024 · Methods in molecular biology
  23. MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells
    Spencer Grissom, Zachary Dixon, Abhyudai Singh, Mark Blenner · 2025 · iScience
  24. CRISPR-based precise methylation of specific FUT8 promoter regions allows isolation of CHO cells with a fine-tuned glycoprofile
    Víctor Jiménez Lancho, Klaus Leitner, Kitty Agarwal, Arathi M. Krishnakumar, Anurag Khetan, Nicole Borth, Nicolas Marx · 2026 · Journal of Biotechnology
  25. Pioneer Factor FOXA1 Boosts CHO Cell Productivity
    Sienna P. Butterfield, Fay L. Saunders, Robert J. White · 2026 · Biotechnology Journal
  26. Intracellular Lactate Modulation Alters Histone Modifications and CHO Cell Performance in Fed‐Batch Culture
    Mona Hoseini Soflaee, Ricardo Romero-Moreno, Bethany Cheng, Nathaniel Chavez, Hui‐Lan Hu, Derek Sha, Michael W. Handlogten · 2026 · Biotechnology and Bioengineering
  27. Expression Systems for Recombinant Biopharmaceutical Production by Mammalian Cells in Culture
    Adam J. Brown, Devika Kalsi, Alejandro Fernandez‐Martell, Joe Cartwright, Nicholas Barber, Yash D. Patel, Richard Turner, Claire L. Bryant, Yusuf B. Johari, David C. James · 2017 · Methods and principles in medicinal chemistry
  28. Toward Integrated Multi‐omics Analysis for Improving CHO Cell Bioprocessing
    Kok Siong Ang, Jong Kwang Hong, Meiyappan Lakshmanan, Dong‐Yup Lee · 2019 · Wiley eBooks
  29. Restoration of deficient DNA Repair Genes Mitigates Genome Instability and Increases Productivity of Chinese Hamster Ovary Cells
    Philipp N. Spahn, Xiaolin Zhang, Qing Hu, Nathaniel K. Hamaker, Hooman Hefzi, Shangzhong Li, Chih‐Chung Kuo, Yingxiang Huang, Jamie Lee, Peter Ly et al. · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
  30. Chromosomal Instability Drives Convergent and Divergent Evolution Traits in Mammalian CHO Lineages
    Steven C. Huhn, Mei‐Ping Chang, Amit Kumar, Ren Liu, Bo Jiang, Michael J. Betenbaugh, Henry Lin, Gregg B. Nyberg, Zhimei Du · 2021 · SSRN Electronic Journal
  31. Identification of novel promoters and genetic control elements derived from Chinese Hamster Ovary cells
    N.L. Nguyen, Martina Baumann, Heena Dhiman, Inmaculada Hernandez Lopez, Nicole Borth · 2018 · New Biotechnology
  32. Engineering and Delivery of Synthetic Chromatin Effectors
    Stefan J. Tekel · 2019
  33. Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
    Sofie A. O’Brien · 2020 · University of Minnesota Digital Conservancy (University of Minnesota)
  34. Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure
    Lyubov A. Yarinich, Anna A. Ogienko, Alexey V. Pindyurin, Evgeniya S. Omelina · 2023 · Vavilov Journal of Genetics and Breeding
  35. Additional file 1 of Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures
    Dylan G. Chitwood, Qinghua Wang, Elliott, Kathryn, Aiyana Bullock, Dwon Jordana, Zhigang Li, Cathy H. Wu, Sarah W. Harcum, Christopher A. Saski · 2021 · TigerPrints (Clemson University)
  36. Additional file 2 of Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures
    Dylan G. Chitwood, Qinghua Wang, Elliott, Kathryn, Aiyana Bullock, Dwon Jordana, Zhigang Li, Cathy H. Wu, Sarah W. Harcum, Christopher A. Saski · 2021 · TigerPrints (Clemson University)
  37. Heritable non-genetic phenotypes are enriched for stress responses as a form of bet hedging
    Spencer Grissom, Zachary Dixon, Abhyudai Singh, Mark Blenner · 2024 · bioRxiv (Cold Spring Harbor Laboratory)
  38. Machine Learning-based Prediction of the Long-term Stability of Chinese Hamster Ovary Cells due to Epigenetic Changes
    Pedro Seber, Richard D. Braatz · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  39. Pioneer factor FOXA1 can boost CHO cell productivity
    Sienna P. Butterfield, Fay L. Saunders, Robert J. White · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  40. Whole-genome DNA methylation analysis of Chinese hamster ovary cells undergoing media adaptation
    Suki Roy, Jasrene Kaur Sandhu, Lingzhi Huang, Abraham Wong, Guo Xuan Wan, Frank Lyko, Emeka Ignatius Igwe, Florian Böhl · 2026 · Frontiers in Bioengineering and Biotechnology
  41. Evaluation of gene editing in CHO cells using the Cas‐ CLOVER system
    Tiffany A. McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, José Miguel Escandell, Mafalda M. Dias, Iona McCall et al. · 2026 · Biotechnology Progress
  42. Genome-Scale Big Data boostet die Produktion von Biotherapeutika
    Nicole Borth · 2026 · BIOspektrum
  43. Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived CHO cell line
    Theodore W. Peters, Jake T. Leinas, Sofie O'Brien, Helena Ochoa‐Moreno, Daniel M. Lachance, Patrick Younan, Gregory Lee Szeto, Howard R. G. Clarke · 2026 · Biotechnology Progress
  44. Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line
    Shu-Min Zhang, Michele Shannon, Wendy M Halsey, Holly Ruess, Christopher Traini, Zhaohui Ao, Xiaodan Ji, Yuan Zhu, Kathleen E. Van Manen-Brush, Andrew Thomson et al. · 2026 · BioTechniques
  45. Paving the Way for a Minimal CHO ChassisExploring the Upper Limits of Megabase-Scale Deletions through DNA Repair Modulation
    Melina Bräuer, Emely Walker, Stefan Schneider, Linus Weiß, Anna Geiger, Benjamin Lindner, Oliver Hädicke, Moritz Johannes Schmidt, Simon Fischer, Kerstin Otte · 2026 · ACS Synthetic Biology
  46. Improving recombinant protein productivity in CHO cells via multi-omics data integration
    Yuan Shen, Lei Shi, Xi Zhang, Xiao Guo, Wei-hua Dong, Tianyun Wang · 2026 · Bioresources and Bioprocessing
  47. Global structure and determinants of transcriptomic adaptation in CHO cells
    Markus Riedl, Nicolas Marx, Nicole Borth · 2026 · New Biotechnology
  48. Same hydrolase knockouts, different outcomes: CRISPR/Cas9 editing workflow shapes resulting cellular phenotype and transcriptome in CHO
    Linus Weiß, Stefan Schneider, Kerstin Otte, Simon Fischer · 2026 · Frontiers in Bioengineering and Biotechnology