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Diffusion coefficients of glucose and ethanol in cell‐free and cell‐occupied calcium alginate membranes

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Hannoun, Betty J. M., Stephanopoulos, Gregory·1986·Biotechnology and Bioengineering

Abstract

The diffusivities of glucose and ethanol in cell‐free and cell‐occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol concentrations had no effect on the respective diffusion coefficients. The ratio of ethanol diffusivity to glucose diffusivity in 2 and 4% alginate agreed closely with the inverse ratio of the hydrodynamic raii for the two molecules in water, indicating that the hydrodynamic theory of diffusion in liquids may be applicable to diffusion in dilute alginate gels. Also, the presence of 20% dead yeast cells had no effect on the diffusivities. The data reported can be used to study reaction and diffusion in immobilized cell reactors and cell physiology under immobilized conditions.

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Metadata

Authors
Hannoun, B. J. M., & Stephanopoulos, G.
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
1986-06
Volume
28
Issue
6
Pages
829-835
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
OpenAlex
W2161016084
Cited by
217
References
0
FWCI
6.38
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Diffusion coefficients of glucose and ethanol in cell‐free and cell‐occupied calcium alginate membranes. https://app.cochat.ai/papers/10.1002/bit.260280609
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Hannoun, B. J. M., & Stephanopoulos, G. (1986). Diffusion coefficients of glucose and ethanol in cell‐free and cell‐occupied calcium alginate membranes. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.260280609

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