The DOI matches its Crossref record.
Induction of neural differentiation in rat C6 glioma cells with taxol
Abstract
Background: Glioblastoma is a common and aggressive type of primary brain tumor. Several anticancer drugs affect GBM (glioblastoma multiforme) cells on cell growth and morphology. Taxol is one of the widely used antineoplastic drugs against many types of solid tumors, such as breast, ovarian, and prostate cancers. However, the effect of taxol on GBM cells remains unclear and requires further investigation. Methods: Survival rate of C6 glioma cells under different taxol concentrations was quantified. To clarify the differentiation patterns of rat C6 glioma cells under taxol challenge, survived glioma cells were characterized by immunocytochemical, molecular biological, and cell biological approaches. Results: After taxol treatment, not only cell death but also morphological changes, including cell elongation, cellular processes thinning, irregular shapes, and fragmented nucleation or micronuclei, occurred in the survived C6 cells. Neural differentiation markers NFL (for neurons), βIII‐tubulin (for neurons), GFAP (for astrocytes), and CNP ase (for oligodendrocytes) were detected in the taxol‐treated C6 cells. Quantitative analysis suggested a significant increase in the percentage of neural differentiated cells. The results exhibited that taxol may trigger neural differentiation in C6 glioma cells. Increased expression of neural differentiation markers in C6 cells after taxol treatment suggest that some anticancer drugs could be applied to elimination of the malignant cancer cells as well as changing proliferation and differentiation status of tumor cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The pattern of GFAP upregulation by LDL is consistent with previous reports that deleterious stimuli induce GFAP expression in C6 cells, supporting the interpretation that LDL triggers a classical astrocytic stress response.
“This pattern is consistent with previous reports showing that deleterious stimuli induce GFAP expression in C6 cells (Shu et al. 2011; Chao et al. 2015), supporting the interpretation that LDL triggers a classical astrocytic stress response.”
From LDL Cholesterol Modulates Astrocyte Metabolism, Lipid Handling, and Morphology: Evidence From In Vitro and In Vivo Models (2026) · cites this paper as Chao et al. 2015
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 41
- Abramovitch R., 1995, Neovascularization induced growth of implanted C6 glioma multicellular spheroids: magnetic resonance microimaging, Cancer Res., 55, 1956
- 10.1017/S0317167100043468 · DOI
- 10.1126/science.161.3839.370 · DOI
- Bernstein J. J., 1990, C6 glioma cell invasion and migration of rat brain after neural homografting: ultrastructure, Neurosurgery, 26, 622, 10.1227/00006123-199004000-00010 · DOI
- Bernstein J. J., 1991, C6 glioma‐astrocytoma cell and fetal astrocyte migration into artificial basement membrane: a permissive substrate for neural tumors but not fetal astrocytes, Neurosurgery, 28, 652, 10.1227/00006123-199105000-00003 · DOI
- 10.1016/j.neuroscience.2007.11.055 · DOI
- Caccamo D., 1989, Immunohistochemistry of a spontaneous murine ovarian teratoma with neuroepithelial differentiation. Neuron‐associated beta‐tubulin as a marker for primitive neuroepithelium, Lab. Invest., 60, 390
- 10.3171/jns.1995.83.4.0665 · DOI
- 10.1016/S0304-3835(01)00404-9 · DOI
- 10.1007/BF01611439 · DOI
- 10.1002/cm.10132 · DOI
- Horwitz S. B., 1994, Taxol (paclitaxel): mechanisms of action, Ann. Oncol., 5, S3
- 10.1007/s10571-007-9229-y · DOI
- 10.1016/j.canlet.2012.08.004 · DOI
- 10.1002/cncr.23223 · DOI
- 10.1177/088307380301801205 · DOI
- 10.1007/s11064-007-9321-1 · DOI
- 10.1038/nrc2803 · DOI
- 10.1172/JCI119642 · DOI
- 10.1038/sj.bjc.6690507 · DOI
- 10.1016/j.lungcan.2011.03.017 · DOI
- 10.1002/neu.10270 · DOI
- 10.1111/j.1365-2559.2007.02792.x · DOI
- 10.1016/j.bbrc.2010.09.098 · DOI
- 10.1073/pnas.74.10.4501 · DOI
- 10.1111/j.1471-4159.1983.tb04768.x · DOI
- 10.1111/j.1471-4159.1992.tb10948.x · DOI
- 10.3109/1547691X.2011.642418 · DOI · Paper page
- 10.1007/BF00334878 · DOI
- 10.1210/en.2004-0127 · DOI
- Ranganathan S., 1996, Increase of beta(III)‐ and beta(IVa)‐tubulin isotopes in human prostate carcinoma cells as a result of estramustine resistance, Cancer Res., 56, 2584
- 10.1016/S0167-4781(97)00168-1 · DOI
- 10.1007/BF00172924 · DOI
- 10.3171/jns.1971.34.3.0335 · DOI
- 10.1038/35097581 · DOI
- 10.1074/jbc.M311844200 · DOI
- Umezu T., 2008, Taxol resistance among the different histological subtypes of ovarian cancer may be associated with the expression of class III beta‐tubulin, Int. J. Gynecol. Pathol., 27, 207
- 10.1007/s10495-006-0007-y · DOI
- 10.1073/pnas.0603704103 · DOI · Paper page
- 10.1016/S0169-328X(96)00239-2 · DOI
- 10.1002/ijc.25975 · DOI
Cited by
Sorted by citations
Baumart, G. J., Rodrigues, M. S., Sholl, J. N., de Sousa, A. C., Weber, A. F., Costa‐Beber, L. C., Farias, H. R., Costa, M. V., Peres, A. M., de Camargo, P. R., et al. (2026) · Journal of Neurochemistry · doi:10.1111/jnc.70403 · Cited by 2
Metadata
- Authors
- Chao, C., Kan, D., Lo, T., Lu, K., & Chien, C.
- Affiliations
- Department of Anatomy and Cell Biology College of Medicine National Taiwan University Taipei Taiwan
- Center of Genomic Medicine National Taiwan University Taipei Taiwan
- Venue
- Brain and Behavior
- Publisher
- Wiley
- Published
- 2015-10-26
- Volume
- 5
- Issue
- 12
- ISSN
- 2162-3279
- PMC
- PMC4667627
- Cited by
- 28
- References
- 0
- Language
- en
- Funders
- Ministry of Science and Technology Taiwan (NSC 100-2320-B-002-085)
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
