The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Rapid Flow‐Based Peptide Synthesis
Open accessAbstract
A flow‐based solid‐phase peptide synthesis methodology that enables the incorporation of an amino acid residue every 1.8 min under automatic control or every 3 min under manual control is described. This is accomplished by passing a stream of reagent through a heat exchanger into a low volume, low backpressure reaction vessel, and through a UV detector. These features enable continuous delivery of heated solvents and reagents to the solid support at high flow rate, thereby maintaining maximal concentration of reagents in the reaction vessel, quickly exchanging reagents, and eliminating the need to rapidly heat reagents after they have been added to the vessel. The UV detector enables continuous monitoring of the process. To demonstrate the broad applicability and reliability of this method, it was employed in the total synthesis of a small protein, as well as dozens of peptides. The quality of the material obtained with this method is comparable to that for traditional batch methods, and, in all cases, the desired material was readily purifiable by RP‐HPLC. The application of this method to the synthesis of the 113‐residue Bacillus amyloliquefaciens RNase and the 130‐residue DARPin pE59 is described in the accompanying manuscript.
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Cites
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- 2000, Fmoc Solid‐Phase Peptide Synthesis: A Practical Approach, Vol. 222
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The greening of peptide synthesis
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Varró, N., Mándityné Huszka, B., Erdei, E., Mándoki, A., & Mándity, I. M. (2025) · Chemistry–Methods · doi:10.1002/cmtd.202500010 · Cited by 8
Metadata
- Authors
- Simon, M. D., Heider, P. L., Adamo, A., Vinogradov, A. A., Mong, S. K., Li, X., Berger, T., Policarpo, R. L., Zhang, C., Zou, Y., Liao, X., Spokoyny, A. M., Jensen, K. F., & Pentelute, B. L.
- Affiliations
- Massachusetts Institute of Technology (US)
- Corresponding author
- Pentelute, Bradley L.
- ORCID
- Venue
- ChemBioChem
- Publisher
- Wiley
- Published
- 2014-03-11
- Volume
- 15
- Issue
- 5
- Pages
- 713-720
- ISSN
- 1439-4227 (ISSN-L)
- 1439-7633
- PMC
- PMC4045704
- OpenAlex
- W1508435089
- Cited by
- 4
- References
- 30
- Citations by year
- 2024: 1 · 2021: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- ChemBioChem (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- PMC
- Funders
- National Institute of General Medical Sciences (T32GM008334, F32GM101762)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Innovative Microfluidic and Catalytic Techniques Innovation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- solid-phase peptide synthesis, heat exchangers, UV monitoring, reagent delivery, peptide purification
- MeSH terms
- Amino Acid Sequence, Equipment Design, Molecular Sequence Data, Peptides, Time Factors, Solid-Phase Synthesis Techniques
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