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Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis
Abstract
Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate‐binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure‐guided site‐directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis.
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Cites
A bacterium that degrades and assimilates poly(ethylene terephthalate)
Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y., & Oda, K. (2016) · Science · doi:10.1126/science.aad6359 · Cited by 3,367
Cited by
Cui, Y., Chen, Y., Liu, X., Dong, S., Tian, Y., Qiao, Y., Mitra, R., Han, J., Li, C., Han, X., et al. (2021) · ACS Catalysis · doi:10.1021/acscatal.0c05126 · Cited by 580
Self-citationStructure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate
Palm, G. J., Reisky, L., Böttcher, D., Müller, H., Michels, E. A. P., Walczak, M. C., Berndt, L., Weiss, M. S., Bornscheuer, U. T., & Weber, G. (2019) · Nature Communications · doi:10.1038/s41467-019-09326-3 · Cited by 491
Enzyme discovery and engineering for sustainable plastic recycling
Zhu, B., Wang, D., & Wei, N. (2022) · Trends in Biotechnology · doi:10.1016/j.tibtech.2021.02.008 · Cited by 366
Kawai, F., Kawabata, T., & Oda, M. (2020) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.0c01638 · Cited by 259
Metadata
- Authors
- Liu, B., He, L., Wang, L., Li, T., Li, C., Liu, H., Luo, Y., & Bao, R.
- Venue
- ChemBioChem
- Publisher
- Wiley
- Published
- 2018-05-28
- Volume
- 19
- Issue
- 14
- Pages
- 1471-1475
- ISSN
- 1439-4227 (ISSN-L)
- 1439-7633
- OpenAlex
- W2795284646
- Cited by
- 210
- References
- 1
- FWCI
- 7.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- Liu, B., He, L., Wang, L., Li, T., Li, C., Liu, H., Luo, Y., & Bao, R. (2018). Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis. ChemBioChem. https://doi.org/10.1002/cbic.201800097
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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