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Cited by 1Lipid Membrane Structure and BehaviorCellular Mechanics and InteractionsCaveolin-1 and cellular processes

Instant Membrane Stabilization by Damage‐Triggered Actin Polymerization in Giant Unilamellar Vesicles

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Nguyen, Huong Thanh, Lee, Sang Ho, Kim, Chang Ho, Shin, Kwanwoo·2026·ChemBioChem
DOI 10.1002/cbic.202500974PMC PMC13000367
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Abstract

Giant unilamellar vesicles (GUVs) rupture catastrophically when membrane pores expand under tension, because they lack cellular repair machinery. Here we present a minimal, damage‐triggered stabilization mechanism based on rapid actin polymerization inside GUVs. ATP‐activated G‐actin was encapsulated in a divalent‐cation‐free lumen while high concentrations of Ca 2+ or Mg 2+ were supplied externally, creating a condition where membrane poration immediately drives cation influx. Upon poration, localized cation entry induces fast F‐actin assembly that forms a cortical‐like meshwork at the defect, mechanically arresting pore enlargement and preserving vesicle morphology for extended periods. The resulting actin plug does not restore full impermeability to small molecules, but it substantially increases vesicle survival against rupture and recapitulates the “plugging” aspect of single‐cell membrane wound responses in a bottom‐up artificial cell model.

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  • Human red blood cell membrane stabilisation is indicative of anti-inflammatory activity.

    “Human red blood cells (HRBCs) were used to evaluate membrane stabilisation potential, which is indicative of anti-inflammatory activity (Nguyen et al., 2026;Pinto et al., 2017;Utterström et al., 2022;Zhang et al., 2026).”

    From PHYTOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTI-INFLAMMATORY, AND CYTOTOXIC ACTIVITIES OF SEQUENTIAL EXTRACTS FROM RIPE BANANA (MUSA PARADISIACA L.) PEEL (2026) · cites this paper as Nguyen et al., 2026

    The Results section on actin-based stabilization examines membrane damage in giant unilamellar vesicles, not human red blood cells. Searches for HRBCs, erythrocytes, hemolysis, and anti-inflammatory activity found no passage linking red blood cell membrane stabilization to anti-inflammatory activity. No page number was available in our copy. (LLM, conf=0.76)

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Research works printed but not linked: 29

  1. [1]D. A. Ammendolia, W. M. Bement, and J. H. Brumell, “ Plasma Membrane Integrity: Implications for Health and Disease,” BMC Biology 19 (2021): 71. 33849525 PMC8042475
  2. [2]S. K. Y. Tang and W. F. Marshall, “ Self‐Repairing Cells: How Single Cells Heal Membrane Ruptures and Restore Lost Structures,” Science 356 (2017): 1022– 1025. 28596334 PMC5664224
  3. [3]C. Dias and J. Nylandsted, “ Plasma Membrane Integrity in Health and Disease: Significance and Therapeutic Potential”, Cell Discovery 7 (2021): 4. 33462191 PMC7813858
  4. [4]M. Nakamura, A. N. M. Dominguez, J. R. Decker, A. J. Hull, J. M. Verboon, and S. M. Parkhurst, “ In to the Breach: How Cells Cope with Wounds,” Open Biology 8 (2018): 180135. 30282661 PMC6223217
  5. [5]A. Horn and J. K. Jaiswal, “ Cellular Mechanisms and Signals that Coordinate Plasma Membrane Repair,” Cellular and Molecular Life Sciences 75 (2018): 3751– 3770. 30051163 PMC6541445
  6. [6]A. M. Moe, A. E. Golding, and W. M. Bement, “ Cell Healing: Calcium, Repair and Regeneration,” Seminars in Cell & Developmental Biology 45 (2015): 18– 23. 26514621 PMC4849125
  7. [7]M. Schuh, “ An Actin‐Dependent Mechanism for Long‐Range Vesicle Transport,” Nature Cell Biology 13 (2011): 1431– 1436. 21983562 PMC3783939
  8. [8]E. M. Vaughan, J. S. You, H. Y. Yu, et al., “ Lipid Domain–dependent Regulation of Single‐Cell Wound Repair,” Molecular Biology of the Cell 25 (2014): 1867– 1876. 24790096 PMC4055266
  9. [9]X. Meng, Q. Yang, X. Yu, et al., “ Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin‐2 and EFF‐1 to Promote Membrane Repair in C. elegans,” Developmental Cell 54 (2020): 624– 638. 32668210
  10. [10]J. K. Jaiswal, S. P. Lauritzen, L. Scheffer, et al., “ S100A11 Is Required for Efficient Plasma Membrane Repair and Survival of Invasive Cancer Cells,” Nature Communications 5 (2014): 3795. PMC4026250 24806074
  11. [11]A. R. Demonbreun, M. Quattrocelli, D. Y. Barefield, M. V. Allen, K. E. Swanson, and E. M. McNally, “ An Actin‐Dependent Annexin Complex Mediates Plasma Membrane Repair in Muscle,” The Journal of Cell Biology 213 (2016): 705– 718. 27298325 PMC4915191
  12. [12]S. Xu and A. D. Chisholm, “ A Gαq‐Ca2+ Signaling Pathway Promotes Actin‐Mediated Epidermal Wound Closure in C. elegans,” Current Biology 21 (2011): 1960– 1967. 22100061 PMC3237753
  13. [13]A. D. Blazek, B. J. Paleo, and N. Weisleder, “ Membrane Repair: A Matter of Life or Dead for Muscle Cells,” Physiology (Bethesda) 30 (2015): 438– 448. PMC4630197 26525343
  14. [14]E. Karatekin, O. Sandre, H. Guitouni, N. Borghi, P.‐H. Puech, and F. Brochard‐Wyart, “ Cascades of Transient Pores in Giant Vesicles: Line Tension and Transport,” Biophysical Journal 84 (2003): 1734– 1749. 12609875 PMC1302742
  15. [15]M.‐T. Lee, W.‐C. Hung, F.‐Y. Chen, and H. W. Huang, “ Mechanism and Kinetics of Pore Formation in Membranes by Water‐Soluble Amphipathic Peptides,” Proceedings of the National Academy of Sciences 105 (2008): 5087– 5092. PMC2278198 18375755
  16. [16]F. Brochard‐Wyart, P. G. de Gennes, and O. Sandre, “ Transient Pores in Stretched Vesicles: Role of Leak‐Out,” Physica A: Statistical Mechanics and its Applications 278 (2000): 32– 51.
  17. [17]O. Sandre, L. Moreaux, and F. Brochard‐Wyart, “ Dynamics of Transient Pores in Stretched Vesicles,” Proceedings of the National Academy of Sciences of the United States of America 96 (1999): 10591– 10596. PMC17927 10485870
  18. [18]R. B. Lira, F. S. C. Leomil, R. J. Melo, K. A. Riske, and R. Dimova, “ To Close or to Collapse: The Role of Charges on Membrane Stability upon Pore Formation,” Advanced Science 8 (2021): 2004068. 34105299 PMC8188222
  19. [19]V. N. Ngassam, W.‐C. Su, D. L. Gettel, et al., “ Recurrent Dynamics of Rupture Transitions of Giant Lipid Vesicles at Solid Surfaces,” Biophysical Journal 120 (2021): 586– 597. 33460597 PMC7896033
  20. [20]C. Hamai, P. S. Cremer, and S. M. Musser, “ Single Giant Vesicle Rupture Events Reveal Multiple Mechanisms of Glass‐Supported Bilayer Formation,” Biophysical Journal 92 (2007): 1988– 1999. 17189305 PMC1861791
  21. [21]A. Granéli, J. Rydström, B. Kasemo, and F. Höök, “ Formation of Supported Lipid Bilayer Membranes on SiO2 from Proteoliposomes Containing Transmembrane Proteins,” Langmuir 19 (2003): 842– 850.
  22. [22]E. Rideau, R. Dimova, P. Schwille, F. R. Wurm, and K. Landfester, “ Liposomes and Polymersomes: A Comparative Review towards Cell Mimicking,” Chemical Society Reviews 47 (2018): 8572– 8610. 30177983
  23. [23]M. T. Abreu‐Blanco, J. M. Verboon, and S. M. Parkhurst, “ Cell Wound Repair in Drosophila Occurs through Three Distinct Phases of Membrane and Cytoskeletal Remodeling,” The Journal of Cell Biology 193 (2011): 455– 464. 21518790 PMC3087011
  24. [24]M. Bovellan, Y. Romeo, M. Biro, et al., “ Cellular Control of Cortical Actin Nucleation,” Current Biology 24 (2014): 1628– 1635. 25017211 PMC4110400
  25. [25]P. Chugh and E. K. Paluch, “ The Actin Cortex at a Glance,” Journal of Cell Science 131 (2018): jcs186254. 30026344 PMC6080608
  26. [26]F. Cavanna and J. Alvarado, “ Quantification of the Mesh Structure of Bundled Actin Filaments,” Soft Matter 17 (2021): 5034– 5043. 33912871
  27. [27]K. Jahnke and K. Gopfrich, “ Engineering DNA‐Based Cytoskeletons for Synthetic Cells,” Interface Focus 13 (2023): 20230028. 37577007 PMC10415745
  28. [28]Z.‐H. Luo, C. Chen, Q.‐H. Zhao, and N.‐N. Deng, “ Functional Metal‐Phenolic Cortical Cytoskeleton for Artificial Cells,” Science Advances 10 (2024), eadj4047. 38363847 PMC10871533
  29. [29]D.‐Y. Li, Z.‐H. Zhou, Y.‐L. Yu, and N.‐N. Deng, “ Microfluidic Construction of Cytoskeleton‐Like Hydrogel Matrix for Stabilizing Artificial Cells,” Chemical Engineering Science 264 (2022): 118186.

Cited by

Sorted by citations

Authors
Nguyen, H. T., Lee, S. H., Kim, C. H., & Shin, K.
Affiliations
Sogang University (KR)
Corresponding author
Shin, Kwanwoo
ORCID
Venue
ChemBioChem
Publisher
Wiley
Published
2026-03-19
Volume
27
Issue
6
Pages
e202500974-e202500974
ISSN
  • 1439-4227 (ISSN-L)
  • 1439-7633
DOI
10.1002/cbic.202500974
PMC
PMC13000367
OpenAlex
W7138862788
Cited by
0
References
0
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • ChemBioChem (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
Funders
Ministry of Education (2018R1A6A1A03024940, 2020R1A6C101A192)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
Keywords
giant unilamellar vesicles, membrane stabilization, membrane rupture, actin polymerization, membrane poration, cation influx, calcium, magnesium, artificial cells
MeSH terms
Actins, Calcium, Cell Membrane, Magnesium, Unilamellar Liposomes, Polymerization

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