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Tubules, Rods, and Spirals: Diverse Modes of SepF ‐ FtsZ Assembling
Published version of a preprint
Abstract
Z‐ring formation by FtsZ, the master assembler of the divisome, is a key step in bacterial cell division. Membrane anchoring of the Z‐ring requires the assistance of dedicated Z‐ring binding proteins, such as SepF and FtsA. SepF participates in bundling and membrane anchoring of FtsZ in gram‐positive bacteria. We report in vitro biophysical studies of the interactions between FtsZ and a cytoplasmic component of cognate SepF from three different bacteria: Mycobacterium tuberculosis , Staphylococcus aureus , and Enterococcus gallinarum . While the cytosolic domain of SepF from M. tuberculosis is primarily a dimer, those from S. aureus and E. gallinarum polymerize to form ring‐like structures. Mycobacterial SepF helps in the bundling of FtsZ filaments to form thick filaments and large spirals. On the other hand, ring‐forming SepF from the Firmicutes bundle FtsZ into tubules. Our results suggest that the oligomeric form of SepF directs how it bundles FtsZ filaments.
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- 10.1038/s41592‐022‐01488‐1 · DOI · Paper page
Metadata
- Authors
- Choudhury, J., & Chaudhuri, B. N.
- Affiliations
- Institute of Microbial Technology (IN)
- Corresponding author
- Choudhury, Jagrity
- Venue
- Cytoskeleton
- Publisher
- Wiley
- Published
- 2024-12-19
- Volume
- 82
- Issue
- 7
- Pages
- 432-443
- ISSN
- 1949-3584 (ISSN-L)
- 1949-3592
- OpenAlex
- W4405653034
- Cited by
- 3
- References
- 88
- Citations by year
- 2025: 3
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cytoskeleton (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- SepF, FtsZ, Z-ring assembly, bacterial cell division, divisome, FtsZ polymerization, oligomeric state, Mycobacterium tuberculosis, Staphylococcus aureus, Enterococcus gallinarum
- MeSH terms
- Bacterial Proteins, Cytoskeletal Proteins, Mycobacterium tuberculosis, Staphylococcus aureus
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- Choudhury, J., & Chaudhuri, B. N. (2025). Tubules, Rods, and Spirals: Diverse Modes of SepF ‐ FtsZ Assembling. Cytoskeleton. https://doi.org/10.1002/cm.21975
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