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Cited by 140Bacterial Genetics and BiotechnologyEscherichia coli research studiesBacterial Identification and Susceptibility Testing

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Growth and Maintenance of Escherichia coli Laboratory Strains

Open access

Abstract

Escherichia coli is a Gram‐negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching‐ or research‐associated laboratory. © 2021 Wiley Periodicals LLC. This article was corrected on 18 July 2022. See the end of the full text for details. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture

Overview

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Integrity signals

Registry

Crossref · 10.1002/cpz1.20

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

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Citations

Cites

Show all 23 references

Research works printed but not linked: 1

  1. 10.1128/JB.185.9.2692.2699.2003 · DOI

Cited by

Metadata

Authors
Tuttle, A. R., Trahan, N. D., & Son, M. S.
Affiliations
Plymouth State University (US)
Corresponding author
Son, Mike S.
Venue
Current Protocols
Publisher
Wiley
Published
2021-01
Volume
1
Issue
1
Pages
e20-e20
ISSN
2691-1299 (ISSN-L)
OpenAlex
W3122438247
Cited by
140
References
23
Citations by year
2026: 37 · 2025: 48 · 2024: 27 · 2023: 24 · 2022: 1 · 2021: 3
FWCI
7.77
Type
journal article
Language
en
Open access
Locations
  • Current Protocols (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Allergy and Infectious Diseases (AI025096, AI039654)
  • National Institute of General Medical Sciences
  • National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords
Escherichia coli, bacterial growth, glycerol storage, liquid culture, growth curves, bacterial enumeration, solid media, laboratory strains
MeSH terms
Agar, Bacteriological Techniques, Culture Media, Escherichia coli, Laboratories

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.