Mesenchymal markers on human adipose stem/progenitor cells
Open accessAbstract
The stromal‐vascular fraction (SVF) of adipose tissue is a rich source of multipotent stem cells. We and others have described three major populations of stem/progenitor cells in this fraction, all closely associated with small blood vessels: endothelial progenitor cells (EPC, CD45−/CD31+/CD34+), pericytes (CD45−/CD31−/CD146+), and supra‐adventitial adipose stromal cells (SA‐ASC, CD45−/CD31−/CD146−/CD34+). EPC are luminal, pericytes are adventitial, and SA‐ASC surround the vessel like a sheath. The multipotency of the pericytes and SA‐ASC compartments is strikingly similar to that of CD45−/CD34−/CD73+/CD105+/CD90+ bone marrow‐derived mesenchymal stem cells (BM‐MSC). Here, we determine the extent to which this mesenchymal pattern is expressed on the three adipose stem/progenitor populations. Eight independent adipose tissue samples were analyzed in a single tube (CD105‐FITC/CD73‐PE/CD146‐PETXR/CD14‐PECY5/CD33‐PECY5/CD235A‐PECY5/CD31‐PECY7/CD90‐APC/CD34‐A700/CD45‐APCCY7/DAPI). Adipose EPC were highly proliferative with (14.3 ± 2.8)% (mean ± SEM) having >2 N DNA. About half (53.1 ± 7.6)% coexpressed CD73 and CD105, and (71.9 ± 7.4)% expressed CD90. Pericytes were less proliferative [(8.2 ± 3.4)% >2 N DNA)] with a smaller proportion [(29.6 ± 6.9)% CD73+/CD105+, (60.5 ± 10.2)% CD90+] expressing mesenchymal associated markers. However, the CD34+ subset of CD146+ pericytes were both highly proliferative [(15.1 ± 3.6)% with >2 N DNA] and of uniform mesenchymal phenotype [(93.3 ± 3.7)% CD73+/CD105+, (97.8 ± 0.7)% CD90+], suggesting transit amplifying progenitor cells. SA‐ASC were the least proliferative [(3.7 ± 0.8)%>2 N DNA] but were also highly mesenchymal in phenotype [(94.4 ± 3.2)% CD73+/CD105+, (95.5 ± 1.2)% CD90+]. These data imply a progenitor/progeny relationship between pericytes and SA‐ASC, the most mesenchymal of SVF cells. Despite phenotypic and functional similarities to BM‐MSC, SA‐ASC are distinguished by CD34 expression. © 2012 International Society for Advancement of Cytometry
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References 52 · Cited by 225
Research works printed but not linked: 1
- [37]Corselli M Chen CW Sun B Yap S Rubin JP Peault B The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells Stem cells and development 2012 21 1299 1308 21861688 PMC3353742
- Authors
- Zimmerlin, L., Donnenberg, V. S., Rubin, J. P., & Donnenberg, A. D.
- Affiliations
- Johns Hopkins University (US)
- University of Pittsburgh (US)
- Corresponding author
- Donnenberg, Albert D.
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2012-11-26
- Volume
- 83A
- Issue
- 1
- Pages
- 134-140
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- PMC
- PMC4157311
- OpenAlex
- W1967029176
- Cited by
- 225
- References
- 52
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 9 · 2023: 12 · 2022: 13 · 2021: 21 · 2020: 17 · 2019: 16 · 2018: 25 · 2017: 23 · 2016: 24 · 2015: 27 · 2014: 13 · 2013: 15 · 2012: 2
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Defense (P30CA047904, W911QY, BC032981, BC032981, BC044784, BC044784)
- Hope Foundation
- Hillman Foundation
- National Institutes of Health
- Cancer Institute, University of Pittsburgh (P30CA047904)
- National Heart, Lung, and Blood Institute (N0-1 HB37165, N01 HB037165)
- National Cancer Institute (R01CA114246, P30CA047904)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- adipose-derived stem cells, stromal vascular fraction, endothelial progenitor cells, pericytes, adipose stromal cells, mesenchymal stem cells, CD73, CD105, CD90, CD146, CD34, CD31, CD45, cell proliferation, multipotency, cell surface markers, adipose tissue
- MeSH terms
- Adipose Tissue, Female, Humans, Phenotype, Stem Cells, Biomarkers, Leukocyte Common Antigens, Antigens, CD34, Platelet Endothelial Cell Adhesion Molecule-1, CD146 Antigen, Mesenchymal Stem Cells
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