Flow-cytometric analysis of rare antigen-specific T cells
Open accessAbstract
The cytometric enumeration and characterization of antigen‐specific lymphocytes, as introduced about 15 years ago, has contributed significantly to our understanding of adaptive immune responses in health and disease. Despite the development of several technologies, allowing to directly or indirectly analyze many aspects of lymphocyte specificity and function, several unresolved issues remain, due to the low frequency of certain antigen‐specific lymphocyte subsets and the complexity of T cell antigen recognition. This is especially true for CD4+ conventional as well as regulatory T cells, which bring major contributions to immune protection and pathology. Here we review the current technologies for the analysis of antigen specific T cells within the physiologic T cell repertoire and with a special focus on recent technologies addressing the analysis of rare antigen‐specific T cell populations including naive and regulatory T cells. © 2013 International Society for Advancement of Cytometry
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CD154+ cells were magnetically enriched from 25 to 50 million PBMCs to detect T cells at low frequencies with a detection limit of 1 in 10^6.
“CD154 + cells were magnetically enriched from 25 to 50 million PBMCs to detect T cells at low frequencies (detection limit = 1 in 10 6 ). 4,42”
From Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigen-specific autoimmune diseases (2024) · cites this paper as [4]
The Antigen-Specific Pre-Enrichment section says CD154 enrichment increases sensitivity to approximately 1 in 10^5–10^6 cells, depending on input-cell number. It does not specify enrichment from 25–50 million PBMCs; the Figure 2 caption instead describes enrichment from 10 million PBMCs. (LLM, conf=0.86)
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References 104 · Cited by 155
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- Authors
- Bacher, P., & Scheffold, A.
- Affiliations
- Miltenyi Biotec GmbH Bergisch Gladbach Germany
- Charité – Universitätsmedizin Berlin (DE)
- Corresponding author
- Scheffold, Alexander
- ORCID
- Scheffold, Alexander
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2013-08
- Volume
- 83A
- Issue
- 8
- Pages
- 692-701
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- OpenAlex
- W1498719741
- Cited by
- 155
- References
- 104
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 9 · 2023: 16 · 2022: 17 · 2021: 10 · 2020: 18 · 2019: 18 · 2018: 9 · 2017: 15 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 4
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Keywords
- antigen-specific T cells, T cell receptor repertoire, regulatory T cells, naive T cells, CD4+ T cells, T cell antigen recognition, T cell enumeration, T cell characterization
- MeSH terms
- Animals, Cell Separation, Flow Cytometry, Humans, Lymphocyte Activation, Receptors, Antigen, T-Cell, Antigens, CD, T-Lymphocytes, Regulatory
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