The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Orchestrating multiplexity in polychromatic science through OMIPs : A decade‐old resource to empower biomedical research
Open accessAbstract
As the optimized multicolor immunofluorescence panel (OMIP) platform entered its 10th anniversary since its launch, the multicolor flow cytometry landscape has changed significantly. Likewise, OMIPs have continuously evolved to cover larger panel sizes, increasing number of subpopulations profiled in a single panel, and new species. After a decade of contributions to the OMIP platform, a review of this collection, summarizing its content and purpose for the research community, is timely and due. This review provides an overview of OMIPs and a presentation of the depth and diversity of this collection of validated panels, with the expectation that readers will take advantage of them to empower and accelerate their research.
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“Until now, nearly 80 OMIPs have been published including also OMIPs for Mass cytometry [44] and Full Spectrum flow cytometry [45] with the aims of (1) reducing the time to develop similar panels and (2) providing a starting point for the development of new panels, or (3) for optimizing existing ones (recent overview in [49]).”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [49]
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Cites
Park, L. M., Lannigan, J., & Jaimes, M. C. (2020) · Cytometry Part A · doi:10.1002/cyto.a.24213 · Cited by 350
Autengruber, A., Gereke, M., Hansen, G., Hennig, C., & Bruder, D. (2012) · European Journal of Microbiology and Immunology · doi:10.1556/eujmi.2.2012.2.3 · Cited by 176
Tsuji, K., Ojima, M., Otabe, K., Horie, M., Koga, H., Sekiya, I., & Muneta, T. (2017) · Cell Transplantation · doi:10.3727/096368917x694831 · Cited by 165
Ferrer‐Font, L., Pellefigues, C., Mayer, J. U., Small, S. J., Jaimes, M. C., & Price, K. M. (2020) · Current Protocols in Cytometry · doi:10.1002/cpcy.70 · Cited by 128
OMIP‐044: 28‐color immunophenotyping of the human dendritic cell compartment
Mair, F., & Prlic, M. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23331 · Cited by 96
OMIP‐017: Human CD4+ helper T‐cell subsets including follicular helper cells
Mahnke, Y. D., Beddall, M. H., & Roederer, M. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22269 · Cited by 72
Staser, K. W., Eades, W. C., Choi, J., Karpova, D., & DiPersio, J. F. (2017) · Cytometry Part A · doi:10.1002/cyto.a.23303 · Cited by 56
Rosa, S. C. D., Carter, D. K., & McElrath, M. J. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22218 · Cited by 54
OMIP‐003: Phenotypic analysis of human memory B cells
Wei, C., Jung, J., & Sanz, I. (2011) · Cytometry Part A · doi:10.1002/cyto.a.21112 · Cited by 51
Fluorochrome choices for multi-color flow cytometry
Flores-Montero, J. A., Kalina, T., Corral-Mateos, A., Sanoja-Flores, L., Pérez‐Andrés, M., Martin-Ayuso, M., Sędek, Ł., Rejlová, K., Mayado, A., Fernandez, P. C., et al. (2019) · Journal of Immunological Methods · doi:10.1016/j.jim.2019.06.009 · Cited by 51
Baumgart, S., Peddinghaus, A., Schulte‐Wrede, U., Mei, H. E., & Grützkau, A. (2016) · Cytometry Part A · doi:10.1002/cyto.a.22894 · Cited by 42
OMIPs—Orchestrating multiplexity in polychromatic science
Roederer, M., & Tárnok, A. (2010) · Cytometry Part A · doi:10.1002/cyto.a.20959 · Cited by 41
OMIP‐007: Phenotypic analysis of human natural killer cells
Eller, M. A., & Currier, J. R. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22033 · Cited by 37
OMIP‐001: Quality and phenotype of Ag‐responsive human T‐cells
Mahnke, Y. D., & Roederer, M. (2010) · Cytometry Part A · doi:10.1002/cyto.a.20944 · Cited by 30
OMIP‐011: Characterization of circulating endothelial cells (CECs) in peripheral blood
Lachmann, R., Lanuti, P., & Miscia, S. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22071 · Cited by 23
Eich, M., Trumpp, A., & Schmitt, S. (2019) · Cytometry Part A · doi:10.1002/cyto.a.23845 · Cited by 23
Dusoswa, S. A., Verhoeff, J., & Garcia‐Vallejo, J. J. (2019) · Cytometry Part A · doi:10.1002/cyto.a.23725 · Cited by 22
OMIP‐045: Characterizing human head and neck tumors and cancer cell lines with mass cytometry
Brodie, T. M., Toševski, V., & Medová, M. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23336 · Cited by 21
OMIP‐041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
Furman, N. E. T., Prabhakara, K. S., Bedi, S. S., Cox, C. S., & Olson, S. D. (2017) · Cytometry Part A · doi:10.1002/cyto.a.23267 · Cited by 20
Preijers, F. W. M. B., Huys, E., & Moshaver, B. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22056 · Cited by 19
Frutoso, M., Mair, F., & Prlic, M. (2020) · Cytometry Part A · doi:10.1002/cyto.a.24230 · Cited by 17
OMIP‐019: Quantification of human γδT‐cells, iNKT‐cells, and hematopoietic precursors
Mahnke, Y. D., Beddall, M. H., & Roederer, M. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22326 · Cited by 13
OMIP‐040: Optimized gating of human prostate cellular subpopulations
Henry, G. H., Loof, N., & Strand, D. W. (2017) · Cytometry Part A · doi:10.1002/cyto.a.23187 · Cited by 7
Jaracz‐Ros, A., Hémon, P., Krzysiek, R., Bachelerie, F., Schlecht‐Louf, G., & Gary‐Gouy, H. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23504 · Cited by 7
Mahnke, Y. D., & Tárnok, A. (2020) · Cytometry Part A · doi:10.1002/cyto.a.24232 · Cited by 7
OMIP‐049: Analysis of Human Myelopoiesis and Myeloid Neoplasms
Zhu, G., Brayer, J. B., Padron, E., et al. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23598 · Cited by 6
OMIP‐012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice
Long, B. R., & Stoddart, C. A. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22074 · Cited by 3
Cited by
Sorted by citations
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531
Metadata
- Authors
- Wang, W., & Creusot, R. J.
- Affiliations
- Columbia University Irving Medical Center (US)
- Columbia Center for Translational Immunology Columbia University Irving Medical Center New York New York USA
- Corresponding author
- Wang, Wei
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2021-06-19
- Volume
- 99
- Issue
- 9
- Pages
- 866-874
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- OpenAlex
- W3169407110
- Cited by
- 5
- References
- 27
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 2 · 2021: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Keywords
- multiparameter flow cytometry, species expansion, cell subpopulations, immunophenotyping
- MeSH terms
- Flow Cytometry, Fluorescent Antibody Technique, Biomedical Research
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