Liver‐specific and high‐level expression of human serum amyloid P component gene in transgenic mice
Abstract
To analyze the regulation of human serum amyloid P component (SAP) gene expression, we have produced seven transgenic mice. The 3.3 kb human SAP genes containing about 0.8 kb of 5′ and 1.5 kb of 3′ flanking region were injected into fertilized eggs of C57BL/6 mice. In five of the seven transgenic mice, human SAP was detected in the sera and serum concentrations were higher than that of human serum in three lines. The human SAP gene was expressed only in the liver. Amounts of human mRNA in the liver and serum concentrations of human SAP were roughly proportional to the copy number of the integrated gene. Human SAP production lowered the serum levels of mouse endogenous SAP. With the intraperitoneal administration of lipopolysaccharide, the mRNA levels in the liver and serum levels of mouse SAP increased several‐fold in both the control and transgenic mice. On the other hand, neither the mRNA nor the serum levels of human SAP increased significantly.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
SAP/PTX2 is primarily produced in the liver in physiological conditions in humans.
“Previous studies have shown that SAP/PTX2 is primarily produced in the liver in physiological conditions in humans81–83.”
From Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis (2025) · cites this paper as [83]
Cited by
Sorted by citations
Targeted pharmacological depletion of serum amyloid P component for treatment of human amyloidosis
Pepys, M. B., Herbert, J., Hutchinson, W. L., Tennent, G. A., Lachmann, H. J., Gallimore, J. R., Lovat, L. B., Bartfai, T., Alanine, A., Hertel, C., et al. (2002) · Nature · doi:10.1038/417254a · Cited by 540 · see citing works
“Pure line C57BL/6 mice transgenic for human SAP were bred in London from embryos of the strain previously reporte”
BackgroundSelf-citationAmyloid P component. A critical review
Pepys, M. B., Booth, D. R., Hutchinson, W. L., Gallimore, J. R., Collins, I. M., & Hohenester, E. (1997) · Amyloid · doi:10.3109/13506129709003838 · Cited by 181 · see citing works
Mizuno, A., Kanno, T., HOSHI, M., Shibata, O., Yano, K., Fujise, N., Kinosaki, M., Yamaguchi, K., Tsuda, E., Murakami, A., et al. (2002) · Journal of Bone and Mineral Metabolism · doi:10.1007/s007740200049 · Cited by 146 · see citing works
Milde, R., Ritter, J. M., Tennent, G. A., Loesch, A., Martínez, F. O., Gordon, S., Pepys, M. B., Verschoor, A., & Helming, L. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.10.065 · Cited by 162 · see citing works
“, 2009) knockout (KO) mice as well as human SAP transgenic mice (Iwanaga et al., 1989) were all on C57BL/6 background.”
BackgroundGillmore, J., Hutchinson, W., Herbert, J., Bybee, A., Mitchell, D., Hasserjian, R., Yamamura, K., Suzuki, M., Sabin, C., & Pepys, M. (2004) · Immunology · doi:10.1111/j.1365-2567.2004.01860.x · Cited by 70 · see citing works
Self-citationBlood proteins from transgenic animal bioreactors.
Luboń, H., Paleyanda, R., Velander, W., & Drohan, W. N. (1996) · Transfusion Medicine Reviews · doi:10.1016/s0887-7963(96)80089-7 · Cited by 51
Cummings, D. M., Benway, T. A., Ho, H., Tedoldi, A., Freitas, M. M. F., Shahab, L., Murray, C. E., Richard-Loendt, A., Brandner, S., Lashley, T., et al. (2017) · Cerebral Cortex · doi:10.1093/cercor/bhx046 · Cited by 43 · see citing works
Mikolajek, H., Kolstoe, S., Pye, V. E., Mangione, P., Pepys, M., & Wood, S. P. (2011) · Journal of Molecular Recognition · doi:10.1002/jmr.1090 · Cited by 36 · see citing works
“, 1987) and SAP (Iwanaga et al., 1989) in mice provides the authentic human proteins but their effects, or lack thereof, in a xenogeneic milieu cannot be reliably assigned to the proteins in humans.”
BackgroundSerum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis
Kumar, S., Song, K., Wang, J., Baghel, M. S., Zeng, Y., Shen, K., Zheng, J., Crane, J., Demehri, S., Gao, P., et al. (2025) · Nature Aging · doi:10.1038/s43587-025-00924-z · Cited by 16 · see citing works
“Previous studies have shown that SAP/PTX2 is primarily produced in the liver in physiological conditions in humans 81–83 .”
Expression of human plasma protein genes in ageing transgenic mice
Bowman, B. H., Yang, F., & Adrian, G. S. (1990) · Bioessays · doi:10.1002/bies.950120703 · Cited by 8
Use of genetically altered mice to study the role of serum amyloid P component in amyloid deposition
Maeda, S. (2003) · Amyloid: Journal of Protein Folding Disorders · doi:10.1080/13506129.2003.12088563 · Cited by 7 · see citing works
Self-citationTransgenic Mouse Model of an Autosomal Dominant Disease: Familial Amyloidotic Polyneuropathy
Yamamura, K., Tsahiro, F., Wakasugi, S., Yi, S., Maeda, S., & Shimada, K. (1990) · doi:10.1007/978-3-642-84224-5_11 · Cited by 3
Self-citationMouse Models of Transthyretin Amyloidosis
Ito, S., & Maeda, S. (2009) · Springer eBooks · doi:10.1007/978-3-642-00646-3_16 · Cited by 1 · see citing works
Self-citationAnimal Models of Amyloid Diseases
Ott, S., & Crowther, D. (2013) · doi:10.1002/9783527654185.ch12
Animal Model as a tool for Medical Genomics
Yamamura, K. (2003) · doi:10.2492/jsir.23.269
Self-citation
- Authors
- Iwanaga, T., Wakasugi, S., Inomoto, T., Uehira, M., Ohnishi, S., Nishiguchi, S., Araki, K., Uno, M., Miyazaki, J., Maeda, S., Shimada, K., & Yamamura, K.
- Affiliations
- Kumamoto University (JP)
- Corresponding author
- Yamamura, Ken‐Ichi
- Venue
- Developmental Genetics
- Publisher
- Wiley
- Published
- 1989-01
- Volume
- 10
- Issue
- 5
- Pages
- 365-371
- ISSN
- 0192-253X (ISSN-L)
- 1520-6408
- OpenAlex
- W2103082307
- Cited by
- 20
- References
- 0
- Citations by year
- 2025: 1 · 2020: 1 · 2017: 1 · 2015: 1 · 2013: 1
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- serum amyloid P component, SAP gene, transgenic mice, liver-specific gene expression, gene expression regulation, gene copy number, lipopolysaccharide, serum protein levels, tissue-specific gene expression
- MeSH terms
- Serum Amyloid P-Component, Animals, Gene Expression Regulation, Humans, Inflammation, Injections, Intraperitoneal, Lipopolysaccharides, Mice, Transgenic, RNA, Messenger, Blotting, Southern, Blotting, Western, Restriction Mapping, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
