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A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody
Abstract
Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3‐NS1/1Ag 4.1. One of the resulting hybrid clones (NA1/34) secreted an antibody that was highly specific for human thymocytes. Eighty‐five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 × 10 4 molecules of HTA1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125 I]‐labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45 000 molecular weight.
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Citations
Cited by
Howard, F. D., Ledbetter, J. A., Wong, J., Bieber, C. P., Stinson, E. B., & Herzenberg, L. A. (1981) · The Journal of Immunology · doi:10.4049/jimmunol.126.6.2117 · Cited by 276
Pei, B., Vela, J. L., Zajonc, D., & Kronenberg, M. (2012) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2011.06435.x · Cited by 33
Metadata
- Authors
- McMichael, A. J., Pilch, J. R., Fabre, J. W., Mason, D. Y., Galfré, G., & Milstein, C.
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 1979-03
- Volume
- 9
- Issue
- 3
- Pages
- 205-210
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W1969783034
- Cited by
- 500
- References
- 0
- FWCI
- 24.85
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. https://app.cochat.ai/papers/10.1002/eji.1830090307
- The paper
- McMichael, A. J., Pilch, J. R., Fabre, J. W., Mason, D. Y., Galfré, G., & Milstein, C. (1979). A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. European Journal of Immunology. https://doi.org/10.1002/eji.1830090307
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