Altered thymocyte migration during experimental acute Trypanosoma cruzi infection: combined role of fibronectin and the chemokines CXCL12 and CCL4
Abstract
We previously showed migration disturbances in the thymus during experimental infection with Trypanosoma cruzi, the causative agent of Chagas disease. These changes were related to the enhanced expression of extracellular matrix ligands and receptors, leading to the escape of immature cells to the periphery. Here, we analyzed the expression and role of selected chemokines (CXCL12 and CCL4) and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection. We found increased chemokine deposition in the thymus of infected mice when compared to controls, accompanied by enhanced co-localization with fibronectin as well as up-regulated surface expression of CXCR4 and CCR5 in thymocytes. We also noticed altered thymocyte migration towards the chemokines analyzed. Such an enhancement was even more prominent when fibronectin was added as a haptotatic stimulus in combination with a given chemokine. Our findings suggest that thymocyte migration results from a combined action of chemokines and extracellular matrix (ECM), which can be altered during pathological conditions such as T. cruzi infection, and may be at the origin of the changes in the T cell repertoire seen in this pathological process.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
T-cell mediated migration of thymocyte toward chemokines was observed following trypanosome infection in human.
“Likewise, T-cell mediated migration of thymocyte toward chemokines was observed following trypanosome infection in human (Mendes-da Cruz et al., 2006).”
From Identification of Candidate Signature Genes and Key Regulators Associated With Trypanotolerance in the Sheko Breed (2019) · cites this paper as Mendes-da Cruz et al., 2006
The citation lists haven’t been fetched yet
References 30 · Cited by 58
- Authors
- Mendes‐da‐Cruz, D. A., Silva, J. S., Cotta‐de‐Almeida, V., & Savino, W.
- Affiliations
- Instituto Oswaldo Cruz (BR)
- Corresponding author
- Savino, Wilson
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 2006-05-24
- Volume
- 36
- Issue
- 6
- Pages
- 1486-1493
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W1985310299
- Cited by
- 58
- References
- 30
- Citations by year
- 2025: 5 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 4
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Arca - Repositório Institucional da Fiocruz (submittedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
- Topics
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- thymocyte migration, Trypanosoma cruzi, Chagas disease, acute infection, fibronectin, extracellular matrix, CXCL12, carbon tetrachloride, CXCR4, CCR5, chemokines, haptotaxis, thymus, T cell receptor repertoire, T cell emigration
- MeSH terms
- Animals, Cell Movement, Extracellular Matrix, Fibronectins, Immunohistochemistry, Mice, Inbred BALB C, RNA, Messenger, T-Lymphocytes, Thymus Gland, Trypanosoma cruzi, Chagas Disease, Macrophage Inflammatory Proteins, Receptors, CCR5, Receptors, CXCR4, Chemokines, CC, Chemokines, CXC, Reverse Transcriptase Polymerase Chain Reaction, Mice, Chemokine CXCL12, Chemokine CCL4
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
