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Cited by 169Salivary Gland Disorders and FunctionsMicroRNA in disease regulationSystemic Lupus Erythematosus Research

Altered miR-146a expression in Sjögren’s syndrome and its functional role in innate immunity

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Pauley, Kaleb M., Stewart, Carol M., Gauna, Adrienne E., Dupre, Lauren C., Kuklani, Riya, Chan, Annie L., Pauley, Brad A., Reeves, Westley H. +2 more·2011·European Journal of Immunology
DOI 10.1002/eji.201040757PMC PMC3760391
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Abstract

MicroRNAs (miRNAs), small non‐coding RNA molecules that post‐transcriptionally regulate gene expression, are known to play key roles in regulating immune responses and autoimmunity. We investigated miR‐146a expression in Sjögren's syndrome (SjS) patients as well as in the SjS‐prone C57BL/6.NOD‐Aec1Aec2 mouse model, to elucidate its involvement in SjS pathogenesis. Expression of miR‐146a was examined in the PBMCs of 25 SjS patients and ten healthy donors, as well as in PBMCs, salivary and lacrimal glands of SjS‐prone mice and WT C57BL/6J mice. Functional assays using THP‐1 human monocytes were conducted to determine the biological roles of miR‐146a in innate immunity. Expression of miR‐146a was significantly increased in SjS patients compared with healthy controls, and was upregulated in the salivary glands and PBMCs of the SjS‐prone mouse at both 8 wk (prior to disease onset) and 20 wk (full‐blown disease) of age. More importantly, functional analysis revealed roles for miR‐146a in increasing phagocytic activity and suppressing inflammatory cytokine production while migration, nitric oxide production and expression of antigen‐presenting/costimulatory molecules are not affected in human monocytic THP‐1 cells. Taken together, our data suggest that abnormal expression/regulation of microRNAs in innate immunity may contribute to, or be indicative of, the initiation and progression of SjS.

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  • Overexpression of miR-146a enhances E. coli uptake by THP-1 cells.

    “Overexpression of miR-146a enhances E. coli uptake by THP-1 cells [154]”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [154]

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Research works printed but not linked: 31

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Authors
Pauley, K. M., Stewart, C. M., Gauna, A. E., Dupre, L. C., Kuklani, R., Chan, A. L., Pauley, B. A., Reeves, W. H., Chan, E. K. L., & Cha, S.
Affiliations
  • University of Florida Health (US)
  • University of Florida (US)
Corresponding author
Cha, Seunghee
ORCID
Venue
European Journal of Immunology
Publisher
Wiley
Published
2011-05-27
Volume
41
Issue
7
Pages
2029-2039
ISSN
  • 0014-2980 (ISSN-L)
  • 1521-4141
DOI
10.1002/eji.201040757
PMC
PMC3760391
OpenAlex
W2082825961
Cited by
169
References
1
Citations by year
2026: 6 · 2025: 5 · 2024: 10 · 2023: 9 · 2022: 10 · 2021: 9 · 2020: 9 · 2019: 8 · 2018: 13 · 2017: 14 · 2016: 10 · 2015: 14 · 2014: 19 · 2013: 14 · 2012: 15
FWCI
11.35
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • European Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • DigitalCommons-Cedarville (Cedarville University) (submittedVersion)
Funders
  • National Institutes of Health
  • National Institute of Dental and Craniofacial Research (DE019644, T32DE007200, R01DE019644, T90DE021990)
Topics
  • Health Sciences › Medicine › Physiology › Salivary Gland Disorders and Functions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
Keywords
miR-146a, Sjögren's syndrome, microRNA expression, innate immunity, autoimmunity, salivary glands, lacrimal gland, phagocytic activity, inflammatory cytokines, THP-1 cells, antigen-presenting molecule
MeSH terms
Adult, Aged, Animals, Chemotaxis, Leukocyte, Female, Gene Expression Regulation, Humans, Immunity, Innate, Leukocytes, Mononuclear, Male, Mice, Inbred C57BL, Middle Aged, Monocytes, Nitric Oxide, Phagocytosis, Salivary Glands, Sjogren's Syndrome, Signal Transduction, Gene Expression, Polymerase Chain Reaction, Cytokines, Mice, Inbred NOD, Antigen Presentation, Gene Silencing, MicroRNAs, Mice, Young Adult

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