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Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics
Abstract
This review presents recent progress in employing hydrophilic interaction liquid chromatography (HILIC) for glycan and glycopeptides analysis. After an introduction of this technique, the following themes are addressed: (i) implementation of HILIC in large‐scale studies for analyzing the human plasma N‐glycome; (ii) the use of HILIC UPLC (ultrahigh pressure liquid chromatography) for fast high‐resolution runs and its successful application with online MS for glycan and glycopeptide analysis; (iii) high‐throughput profiling using HILIC solid‐phase extraction in combination with MS detection; (iv) HILIC sample preparation for CE and CGE; (v) the latest glycoproteomic approaches implementing HILIC separation; (vi) future perspectives of HILIC including its use in large‐scale glycoproteomics studies such as the analysis of entire glycoproteomes at the glycopeptide level.
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Citations
Cites
HILIC analysis of fluorescence-labeled N-glycans from recombinant biopharmaceuticals
Melmer, M., Stangler, T., Schiefermeier, M., Brunner, W., Toll, H., Rupprechter, A., Lindner, W., & Premstaller, A. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-3988-x · Cited by 104
Cited by
Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments
Kailemia, M. J., Ruhaak, L. R., Lebrilla, C. B., & Amster, I. J. (2014) · Analytical Chemistry · doi:10.1021/ac403969n · Cited by 377
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Metadata
- Authors
- Zauner, G., Deelder, A. M., & Wuhrer, M.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 2011-12
- Volume
- 32
- Issue
- 24
- Pages
- 3456-3466
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2013172079
- Cited by
- 192
- References
- 1
- FWCI
- 6.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zauner, G., Deelder, A. M., & Wuhrer, M. (2011). Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics. ELECTROPHORESIS. https://doi.org/10.1002/elps.201100247
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