Epigenetic responses following maternal dietary exposure to physiologically relevant levels of bisphenol A
Open accessAbstract
Animal studies have linked perinatal bisphenol A (BPA) exposure to altered DNA methylation, but little attention is given to analyzing multiple physiologically relevant doses. Utilizing the viable yellow agouti (Avy) mouse, we examine the effects of developmental exposure through maternal diet to 50 ng BPA/kg (n = 14 litters), 50 μg BPA/kg (n = 9 litters), or 50 mg BPA/kg (n = 13 litters) on global and candidate gene methylation at postnatal day 22. Global methylation analysis reveals hypermethylation in tail tissue of a/a and A vy/a offspring across all dose groups compared with controls (n = 11 litters; P < 0.02). Analysis of coat color phenotype replicates previous work showing that the distribution of 50 mg BPA/kg A vy/a offspring shifts toward yellow (P = 0.006) by decreasing DNA methylation in the retrotransposon upstream of the Agouti gene (P = 0.03). Maternal exposure to 50 μg or 50 ng BPA/kg, however, results in altered coat color distributions in comparison with control (P = 0.04 and 0.02), but no DNA methylation effects at the Agouti gene are noted. DNA methylation at the CDK5 activator‐binding protein (CabpIAP) metastable epiallele shows hypermethylation in the 50 μg BPA/kg offspring, compared with controls (P = 0.02). Comparison of exposed mouse liver BPA levels to human fetal liver BPA levels indicates that the three experimental exposures are physiologically relevant. Thus, perinatal BPA exposure affects offspring phenotype and epigenetic regulation across multiple doses, indicating the need to evaluate dose effects in human clinical and population studies. Environ. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.
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Bisphenol A and its conjugated metabolites have been measured in multiple studies.
“Nevertheless, bisphenol A and its conjugated metabolites have been measured in multiple studies (Nahar et al., 2013, 2015; Pusiol et al., 2016; Anderson et al., 2012; Cao et al., 2012; Faulk et al., 2015; Mos covitz et al., 2016; Zhang et al., 2011).”
From Pregnancy-related maternal physiological adaptations and fetal chemical exposure (2023) · cites this paper as Anderson et al., 2012
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- Authors
- Anderson, O. S., Nahar, M. S., Faulk, C., Jones, T. R., Liao, C., Kannan, K., Weinhouse, C., Rozek, L. S., & Dolinoy, D. C.
- Affiliations
- University of Michigan (US)
- New York State Department of Health (US)
- Corresponding author
- Dolinoy, Dana C.
- ORCID
- Venue
- Environmental and Molecular Mutagenesis
- Publisher
- Wiley
- Published
- 2012-03-29
- Volume
- 53
- Issue
- 5
- Pages
- 334-342
- ISSN
- 0893-6692 (ISSN-L)
- 1098-2280
- PMC
- PMC3570056
- OpenAlex
- W2014995891
- Cited by
- 140
- References
- 42
- Citations by year
- 2025: 1 · 2024: 6 · 2023: 5 · 2022: 3 · 2021: 6 · 2020: 10 · 2019: 9 · 2018: 15 · 2017: 14 · 2016: 16 · 2015: 15 · 2014: 19 · 2013: 15 · 2012: 6
- FWCI
- 7.08
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Environmental and Molecular Mutagenesis (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Deep Blue (University of Michigan) (submittedVersion)
- Funders
- National Institutes of Health (ES017885)
- National Institute of Environmental Health Sciences (T32ES007062, ES017524, ES017885, R01ES017524, P30ES017885)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R24HD000836)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Keywords
- bisphenol A, maternal dietary exposure, DNA methylation, epigenetics, agouti gene, retrotransposons, metastable epialleles, dose-response relationship, perinatal exposure, hypermethylation
- MeSH terms
- Bisphenol A Compounds, Animals, Base Sequence, Benzhydryl Compounds, Diet, DNA, Female, Liver, Molecular Sequence Data, Phenols, Pregnancy, Maternal Exposure, DNA Methylation, Epigenesis, Genetic, Mice
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