The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A
Open accessAbstract
We generated a ROSA26-eGFP-DTA mouse line by introducing an eGFP-DTA (enhanced green fluorescent protein -- diphtheria toxin fragment A) cassette into the ROSA26 locus by homologous recombination in ES cells. This mouse expresses eGFP ubiquitously, but DTA expression is prevented by the presence of eGFP, a Neo cassette, and a strong transcriptional stop sequence. Mice carrying this construct are normal and fertile, indicating the absence of DTA expression. However, upon Cre-mediated excision of the floxed region DTA expression is activated, resulting in the specific ablation of Cre-expressing cells. As an example of this approach, we ablated Nkx2.5 and Wnt1-expressing cells by using the Nkx2.5-Cre and Wnt1-Cre mouse lines, respectively. We observed loss of the precise tissues in which Nkx2.5 and Wnt1 are expressed. Apart from being a general GFP reporter, the ROSA26-GFP-DTA mouse line should provide a useful resource for genetic ablation of specific groups of cells.
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Thomas, K. R., & Capecchi, M. R. (1990) · Nature · doi:10.1038/346847a0 · Cited by 896
Cited by
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Lepper, C., Partridge, T. A., & Fan, C.-M. (2011) · Development · doi:10.1242/dev.067595 · Cited by 1,105
Redefining the identity of cardiac fibroblasts
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He, W. A., Berardi, E., Cardillo, V. M., Acharyya, S., Aulino, P., Thomas-Ahner, J., Wang, J., Bloomston, M., Muscarella, P., Nau, P., et al. (2013) · Journal of Clinical Investigation · doi:10.1172/jci68523 · Cited by 357
Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo
Cox, B. C., Chai, R., Lenoir, A., Liu, Z., Zhang, L., Nguyen, D.-H., Chalasani, K., Steigelman, K. A., Fang, J., Cheng, A. G., et al. (2014) · Development · doi:10.1242/dev.103036 · Cited by 285
Metadata
- Authors
- Ivanova, A., Signore, M., Caro, N., Greene, N. D. E., Copp, A. J., & Martinez-Barbera, J. P.
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2005
- Volume
- 43
- Issue
- 3
- Pages
- 129-135
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W2079641406
- Cited by
- 256
- References
- 1
- FWCI
- 2.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Ivanova, A., Signore, M., Caro, N., Greene, N. D. E., Copp, A. J., & Martinez-Barbera, J. P. (2005). In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A. genesis. https://doi.org/10.1002/gene.20162
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