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Cited by 455Gut microbiota and healthInflammatory Bowel DiseaseMicroscopic Colitis

Twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal Crohnʼs disease

Willing, Ben, Halfvarson, Jonas, Dicksved, Johan, Rosenquist, Magnus, Järnerot, Gunnar, Engstrand, Lars, Tysk, Curt, Jansson, Janet K.·2009·Inflammatory Bowel Diseases
DOI 10.1002/ibd.20783
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Abstract

BACKGROUND: Large interindividual variation in the composition of the intestinal microbiota between unrelated individuals has made it challenging to identify specific aspects of dysbiosis that lead to Crohn's disease (CD). METHODS: To reduce variations in exposure during establishment of the gut flora and the influence of genotype, we studied the mucosa-associated microbiota of monozygotic twin pairs that were discordant (n = 6) or concordant (n = 4) for CD. DNA was extracted from biopsies collected from 5 locations between the ileum and rectum. Bacterial 16S ribosomal RNA genes were amplified and community composition assessed by terminal-restriction fragment length polymorphism, cloning and sequencing, and quantitative real-time polymerase chain reaction (PCR). RESULTS: The microbial compositions at all biopsy locations for each individual were similar, regardless of disease state, but there were differences between individuals. In particular, individuals with predominantly ileal CD had a dramatically lower abundance (P < 0.001) of Faecalibacterium prausnitzii and increased abundance (P < 0.03) of Escherichia coli compared to healthy co-twins and those with CD localized in the colon. This dysbiosis was significantly correlated to the disease phenotype rather than genotype. CONCLUSIONS: The reduced abundance of F. prausnitzii and increased abundance of E. coli are indicative of an ileal CD phenotype, distinct from colonic CD, and the relative abundances of these specific bacterial populations are promising biomarker candidates for differential diagnosis of CD and eventually customized treatment.

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  • This dysbiosis correlated more strongly with disease phenotype than with patient genotype.

    “This dysbiosis correlated better with disease phenotype than with the genotype of the patient.22”

    From Probiotics and the gut microbiota in intestinal health and disease (2010) · cites this paper as [22]

  • In twins, lower F. prausnitzii and higher E. coli abundance are associated with an ileal Crohn's disease phenotype.

    “A recent study in twins revealed that a reduced abundance of the commensal bacterium Fecalibacterium prausnitzii and an increased abundance of Escherichia coli species was associated with an ileal Crohn’s disease phenotype.”

    From Probiotics and the gut microbiota in intestinal health and disease (2010) · cites this paper as [22]

  • UC and CD present a concomitant increase in the abundance of Escherichia coli.

    “UC and Crohn´s disease (CD) present a low abundance of F. prausnitzii, which has known anti-inflammatory properties [38] , and this is associated with a higher risk of postoperative recurrence of ileal disease [29] , and with a concomitant increase in the abundance of Escherichia coli (E.coli) [39] .”

    From Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis (2014) · cites this paper as [39]

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Authors
Willing, B., Halfvarson, J., Dicksved, J., Rosenquist, M., Järnerot, G., Engstrand, L., Tysk, C., & Jansson, J. K.
Affiliations
  • Örebro University Hospital (SE)
  • Swedish University of Agricultural Sciences (SE)
  • Uppsala University Hospital (SE)
  • Swedish Institute (SE)
  • Örebro University (SE)
  • Lawrence Berkeley National Laboratory (US)
ORCID
Venue
Inflammatory Bowel Diseases
Publisher
Oxford University Press (OUP)
Published
2009-05
Volume
15
Issue
5
Pages
653-660
ISSN
1078-0998 (ISSN-L)
DOI
10.1002/ibd.20783
OpenAlex
W2034157397
Cited by
455
References
51
Citations by year
2026: 7 · 2025: 5 · 2024: 11 · 2023: 13 · 2022: 17 · 2021: 22 · 2020: 25 · 2019: 23 · 2018: 29 · 2017: 20 · 2016: 46 · 2015: 46 · 2014: 40 · 2013: 36 · 2012: 32
FWCI
11.37
Type
journal article
Language
en
Open access
closed
Locations
Inflammatory Bowel Diseases (publishedVersion)
Funders
  • U.S. Department of Energy (-AC02-05CH11231, 05CH11231, DE AC02 05CH11231, Contract DE-AC02-05CH11231., DE-AC02-)
  • Sveriges Lantbruksuniversitet
  • Lawrence Berkeley National Laboratory (AC02‐05CH11231, DE-AC02-05CH11231, 05CH11231)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Health Sciences › Medicine › Epidemiology › Microscopic Colitis
Keywords
Crohn's disease, ileal Crohn's disease

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