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Cited by 75Bacterial Genetics and BiotechnologyAntibiotic Resistance in BacteriaDrug Transport and Resistance Mechanisms

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Differential regulation of the Tn10‐encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.

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Meier, I., Wray, L. V., Hillen, W.·1988·The EMBO Journal
DOI 10.1002/j.1460-2075.1988.tb02846.xPMC PMC454355
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Abstract

The Tn10-encoded tet transcriptional control sequence consists of bidirectional, overlapping promoters which are superimposed by a tandem operator arrangement. Three mutations have been constructed by oligonucleotide-directed mutagenesis which reduce binding of Tet repressor to either one or both of the tandem tet operators 1000-fold as determined by DNAseI footprinting in vitro. The affinity of Tet repressor for mutant tet operators is not affected by the presence of an already occupied neighbouring wild-type operator, indicating little or no cooperativity. The regulation of the divergently oriented tet promoters PA and PR by the tet operators O1 and O2 and Tet repressor provided in trans is determined using transcriptional fusions of the promoters to lacZ and galK indicator genes located with different polarity on the same plasmid. The results demonstrate that expression of the resistance gene tetA is regulated by Tet repressor bound to either O1 or O2. Expression of the repressor gene tetR is only marginally reduced when Tet repressor is bound to O2. This result is discussed with respect to the double promoter structure found for PR. Occupation of O1 with Tet repressor turns off transcription from PR completely. The implications of these findings on the establishment of tetracycline resistance upon induction are discussed.

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References 29 · Cited by 75

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  1. Beck C. F., Mutzel R., Barbé J., Müller W. A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. J Bacteriol. 1982 May;150(2):633–642. doi: 10.1128/jb.150.2.633-642.1982. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  2. Bertrand K. P., Postle K., Wray L. V., Jr, Reznikoff W. S. Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant. J Bacteriol. 1984 Jun;158(3):910–919. doi: 10.1128/jb.158.3.910-919.1984. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  3. Bertrand K. P., Postle K., Wray L. V., Jr, Reznikoff W. S. Overlapping divergent promoters control expression of Tn10 tetracycline resistance. Gene. 1983 Aug;23(2):149–156. doi: 10.1016/0378-1119(83)90046-x. [DOI] [PubMed] [Google Scholar] · DOI
  4. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  5. Coleman D. C., Foster T. J. Analysis of the reduction in expression of tetracycline resistance determined by transposon Tn10 in the multicopy state. Mol Gen Genet. 1981;182(1):171–177. doi: 10.1007/BF00422786. [DOI] [PubMed] [Google Scholar] · DOI
  6. Hillen W., Gatz C., Altschmied L., Schollmeier K., Meier I. Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions. J Mol Biol. 1983 Sep 25;169(3):707–721. doi: 10.1016/s0022-2836(83)80166-1. [DOI] [PubMed] [Google Scholar] · DOI
  7. Hillen W., Klock G., Kaffenberger I., Wray L. V., Reznikoff W. S. Purification of the TET repressor and TET operator from the transposon Tn10 and characterization of their interaction. J Biol Chem. 1982 Jun 10;257(11):6605–6613. [PubMed] [Google Scholar]
  8. Hillen W., Schollmeier K., Gatz C. Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region. J Mol Biol. 1984 Jan 15;172(2):185–201. doi: 10.1016/s0022-2836(84)80037-6. [DOI] [PubMed] [Google Scholar] · DOI
  9. Hillen W., Schollmeier K. Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Res. 1983 Jan 25;11(2):525–539. doi: 10.1093/nar/11.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  10. Hillen W., Unger B. Binding of four repressors to double-stranded tet operator region stabilizes it against thermal denaturation. Nature. 1982 Jun 24;297(5868):700–702. doi: 10.1038/297700a0. [DOI] [PubMed] [Google Scholar] · DOI
  11. Hochschild A., Ptashne M. Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix. Cell. 1986 Mar 14;44(5):681–687. doi: 10.1016/0092-8674(86)90833-0. [DOI] [PubMed] [Google Scholar] · DOI
  12. Jorgensen R. A., Reznikoff W. S. Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10. J Bacteriol. 1979 Jun;138(3):705–714. doi: 10.1128/jb.138.3.705-714.1979. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  13. Koop A. H., Hartley M. E., Bourgeois S. A low-copy-number vector utilizing beta-galactosidase for the analysis of gene control elements. Gene. 1987;52(2-3):245–256. doi: 10.1016/0378-1119(87)90051-5. [DOI] [PubMed] [Google Scholar] · DOI
  14. Kramer W., Drutsa V., Jansen H. W., Kramer B., Pflugfelder M., Fritz H. J. The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Res. 1984 Dec 21;12(24):9441–9456. doi: 10.1093/nar/12.24.9441. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  15. Lambert P. F., Reznikoff W. S. Use of transcriptional repressors to stabilize plasmid copy number of transcriptional fusion vectors. J Bacteriol. 1985 Apr;162(1):441–444. doi: 10.1128/jb.162.1.441-444.1985. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar] · DOI
  17. McClure W. R. Mechanism and control of transcription initiation in prokaryotes. Annu Rev Biochem. 1985;54:171–204. doi: 10.1146/annurev.bi.54.070185.001131. [DOI] [PubMed] [Google Scholar] · DOI
  18. McMurry L., Petrucci R. E., Jr, Levy S. B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974–3977. doi: 10.1073/pnas.77.7.3974. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  19. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar] · DOI
  20. Oehmichen R., Klock G., Altschmied L., Hillen W. Construction of an E. coli strain overproducing the Tn10-encoded TET repressor and its use for large scale purification. EMBO J. 1984 Mar;3(3):539–543. doi: 10.1002/j.1460-2075.1984.tb01843.x. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  21. Ptashne M., Jeffrey A., Johnson A. D., Maurer R., Meyer B. J., Pabo C. O., Roberts T. M., Sauer R. T. How the lambda repressor and cro work. Cell. 1980 Jan;19(1):1–11. doi: 10.1016/0092-8674(80)90383-9. [DOI] [PubMed] [Google Scholar] · DOI
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI · Paper page
  23. Schneider K., Beck C. F. New expression vectors for identifying and testing signal structures for initiation and termination of transcription. Methods Enzymol. 1987;153:452–461. doi: 10.1016/0076-6879(87)53071-3. [DOI] [PubMed] [Google Scholar] · DOI
  24. Schneider K., Beck C. F. Promoter-probe vectors for the analysis of divergently arranged promoters. Gene. 1986;42(1):37–48. doi: 10.1016/0378-1119(86)90148-4. [DOI] [PubMed] [Google Scholar] · DOI
  25. Wissmann A., Meier I., Wray L. V., Jr, Geissendörfer M., Hillen W. Tn10 tet operator mutations affecting Tet repressor recognition. Nucleic Acids Res. 1986 May 27;14(10):4253–4266. doi: 10.1093/nar/14.10.4253. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  26. Wray L. V., Jr, Jorgensen R. A., Reznikoff W. S. Identification of the tetracycline resistance promoter and repressor in transposon Tn10. J Bacteriol. 1981 Aug;147(2):297–304. doi: 10.1128/jb.147.2.297-304.1981. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  27. Wray L. V., Jr, Reznikoff W. S. Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10. J Bacteriol. 1983 Dec;156(3):1188–1191. doi: 10.1128/jb.156.3.1188-1191.1983. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  28. von Hippel P. H., Bear D. G., Morgan W. D., McSwiggen J. A. Protein-nucleic acid interactions in transcription: a molecular analysis. Annu Rev Biochem. 1984;53:389–446. doi: 10.1146/annurev.bi.53.070184.002133. [DOI] [PubMed] [Google Scholar] · DOI

Metadata

Authors
Meier, I., Wray, L. V., & Hillen, W.
Affiliations
Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
1988-02-01
Volume
7
Issue
2
Pages
567-572
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.1002/j.1460-2075.1988.tb02846.x
PMC
PMC454355
OpenAlex
W154073066
Cited by
75
References
29
Citations by year
2026: 1 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 2 · 2012: 6
FWCI
1.55
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
Tn10 transposon, tetracycline resistance, tetA, TetR, Tet repressor, bidirectional promoter, divergent promoters, transcriptional regulation, cooperativity
MeSH terms
Base Sequence, Cloning, Molecular, DNA Transposable Elements, Escherichia coli, Gene Expression Regulation, Genes, Bacterial, Genes, Regulator, Molecular Sequence Data, Mutation, Operon, R Factors, Tetracycline Resistance, Transcription, Genetic

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