Malvolio, the Drosophila homologue of mouse NRAMP-1 (Bcg), is expressed in macrophages and in the nervous system and is required for normal taste behaviour.
Open accessAbstract
We report the sequence, expression pattern and mutant phenotype of malvolio (mvl), the Drosophila homologue of mammalian natural resistance-associated macrophage proteins (NRAMPs). In the mouse, this novel transporter is encoded by Bcg, a dominant gene that confers natural resistance to intracellular parasites. mvl was identified in a screen for mutants that affect taste behaviour. We show that loss-of-function as well as insertional mutants in mvl display defects in taste behaviour with no alterations in the physiology of the sensory neurons. Activity of the reporter enzyme beta-galactosidase, that reflects the expression pattern of mvl, is seen in mature sensory neurons and in macrophages. The conceptual translation of the mvl cDNA shows a striking similarity (65% identity) with human NRAMP with almost complete identity in a conserved consensus motif found in a number of ATP-coupled transporters. Based on its phenotype and expression pattern as well as its structural similarities to NRAMPs and a nitrate transporter in Aspergillus nidulans, we discuss a possible role for MVL in nitrite/nitrate transport and its implications.
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Homologous proteins in yeast, flies, and zebrafish also transport iron and similar divalent cations.
“Homologous proteins in yeast, 63 flies, 64 and zebrafish 65 also transport iron and similar divalent cations.”
From Forging a field: the golden age of iron biology. (2008) · cites this paper as [64]
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References 56 · Cited by 136
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- Authors
- Rodrigues, V., Cheah, P. Y., Ray, K., & Chia, W.
- Affiliations
- National University of Singapore (SG)
- Venue
- The EMBO Journal
- Published
- 1995-07-01
- Volume
- 14
- Issue
- 13
- Pages
- 3007-3020
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC394361
- OpenAlex
- W261351262
- Cited by
- 136
- References
- 56
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 9 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 4 · 2012: 2
- FWCI
- 2.80
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hubei University of Automotive Technology
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
- Keywords
- Malvolio, BCG, NRAMP, Drosophila, macrophages, sensory neurons, nitrate transporters, gene expression patterns, mutant phenotypes
- MeSH terms
- Natural Resistance-associated Macrophage Protein 1, Solute Carrier Family 11, Member 2, Amino Acid Sequence, Animals, Base Sequence, Behavior, Animal, Carrier Proteins, Chromosome Mapping, Drosophila, Fructose, Gene Expression Regulation, Larva, Macrophages, Membrane Proteins, Molecular Sequence Data, Nervous System, Pupa, RNA, Messenger, Sodium Chloride, Sucrose, Taste, Trehalose, Ion Pumps, Genes, Insect, Sequence Homology, Amino Acid, Genes, Reporter, Cation Transport Proteins, Drosophila Proteins, Iron-Binding Proteins, Mice
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