The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Xer-mediated site-specific recombination in vitro
Open accessAbstract
The Xer site-specific recombination system acts at ColE1 cer and pSC101 psi sites to ensure that these plasmids are in a monomeric state prior to cell division. We show that four proteins, ArgR, PepA, XerC and XerD are necessary and sufficient for recombination between directly repeated cer sites on a supercoiled plasmid in vitro. Only PepA, XerC and XerD are required for recombination at psi in vitro. Recombination at cer and psi in vitro requires negative supercoiling and is exclusively intramolecular. Strand exchange at cer produces Holliday junction-containing products in which only the top strands have been exchanged. This reaction requires the catalytic tyrosine residue of Xer C but not that of XerD. Recombination at psi gives catenated circular resolution products. Strand exchange at psi is sequential. XerC catalyses the first (top) strand exchange to make a Holiday junction intermediate and XerD catalyses the second (bottom) strand exchange.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The 11-kb LIR encoded a tyrosine recombinase that belonged to the integrase family.43 , 44”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [44]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Pedigrees of some mutant strains of Escherichia coli K-12
Bachmann, B. (1972) · Bacteriological Reviews · doi:10.1128/br.36.4.525-557.1972 · Cited by 1,700
Pedigrees of some mutant strains of Escherichia coli K-12.
Bachmann, B. J. (1972) · Bacteriological Reviews · doi:10.1128/mmbr.36.4.525-557.1972 · Cited by 600
The integrase family of site‐specific recombinases: regional similarities and global diversity.
Argos, P., Landy, A., Abremski, K., Egan, J. B., Haggård‐Ljungquist, E., Hoess, R. H., Kahn, M. L., Kalionis, B., Narayana, S. V. L., & Pierson, L. S. (1986) · The EMBO Journal · doi:10.1002/j.1460-2075.1986.tb04229.x · Cited by 496
Summers, D., & Sherratt, D. J. (1984) · Cell · doi:10.1016/0092-8674(84)90060-6 · Cited by 446
Self-citationA novel role for site-specific recombination in maintenance of bacterial replicons
Austin, S. J., Ziese, M., & Sternberg, N. L. (1981) · Cell · doi:10.1016/0092-8674(81)90180-x · Cited by 333
Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12
Blakely, G., May, G., McCulloch, R., Arciszewska, L. K., Burke, M. E., Lovett, S. T., & Sherratt, D. J. (1993) · Cell · doi:10.1016/0092-8674(93)80076-q · Cited by 332
Self-citationCatalysis by site-specific recombinases
Stark, W. M., Boocock, M. R., & Sherratt, D. J. (1992) · Trends in Genetics · doi:10.1016/0168-9525(92)90327-z · Cited by 280
Self-citationEscherichia coli RuvC protein is an endonuclease that resolves the Holliday structure.
Iwasaki, H., Takahagi, M., Shiba, T., Nakata, A., & Shinagawa, H. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb05016.x · Cited by 277
Formation and resolution of recombination intermediates by E. coliRecA and RuvC proteins
Dunderdale, H. J., Benson, F. E., Parsons, C. A., Sharples, G. J., Lloyd, R. G., & West, S. C. (1991) · Nature · doi:10.1038/354506a0 · Cited by 233
Site-specific recombination intermediates trapped with suicide substrates
Nunes-Düby, S. E., Matsumoto, L. H., & Landy, A. (1987) · Cell · doi:10.1016/0092-8674(87)90336-9 · Cited by 223
Research works printed but not linked: 2
- Glansdorff,N. (1987) Biosynthesis of arginine and polyamines. In Neidhardt.F.C. (ed.). Salmonella typhi/lmurilun antd Escheric/hia co/li Cellular-antd Molecul/ar Biology. American Society for Microbiology. Washington DC, pp. 32 1-344.
- Sambrook,J.. Fritsch,E.F. and Maniatis,T. (1989) Moleclular Cloninlg: A Laboratory Manuiial. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Cited by
Sorted by citations
Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ
Kuzminov, A. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.4.751-813.1999 · Cited by 897
Similarities and differences among 105 members of the Int family of site-specific recombinases
Nunes-Düby, S. E., Kwon, H. J., Tirumalai, R. S., Ellenberger, T., & Landy, A. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.2.391 · Cited by 448
Aussel, L., Barre, F., Aroyo, M., Stasiak, A., Stasiak, A. Z., & Sherratt, D. J. (2002) · Cell · doi:10.1016/s0092-8674(02)00624-4 · Cited by 321
“Therefore, the DNA transloThe XerC and XerD recombinases were purified as described elsecase activity of FtsK is not necessary for each cell divi- where (Colloms et al., 1996).”
Self-citationHallet, B., & Sherratt, D. J. (2006) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1997.tb00349.x · Cited by 223
Self-citationCrystal structure of the site‐specific recombinase, XerD
Subramanya, H. S., Arciszewska, L. K., Baker, R. A., Bird, L. E., Sherratt, D. J., & Wigley, D. B. (1997) · The EMBO Journal · doi:10.1093/emboj/16.17.5178 · Cited by 210
Self-citation
Metadata
- Authors
- Colloms, S. D., McCulloch, R., Grant, K., Neilson, L., & Sherratt, D. J.
- Affiliations
- University of Oxford (GB)
- Venue
- The EMBO Journal
- Published
- 1996-03-01
- Volume
- 15
- Issue
- 5
- Pages
- 1172-1181
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC450016
- OpenAlex
- W1538904365
- Cited by
- 136
- References
- 43
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 2 · 2022: 2 · 2020: 4 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 5 · 2013: 3 · 2012: 5
- FWCI
- 8.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Wellcome Trust
- Nuffield Foundation
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- XerC, XerD, ArgR, PEPA, plasmid replication, negative supercoiling, Holliday junction, strand exchange, intramolecular recombination
- MeSH terms
- Base Sequence, Binding Sites, DNA Nucleotidyltransferases, DNA, Superhelical, Escherichia coli, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids, Recombination, Genetic, Substrate Specificity, Integrases, Escherichia coli Proteins, Recombinases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
