Tissue Oxidative Capacity, Fuel Stores and Skeletal Muscle Fatty Acid Composition In Obesity‐Prone and Obesity‐Resistant Rats
Abstract
The purpose of the present study was to compare tissue oxidative capacity, skeletal muscle fatty acid composition, and tissue fuel stores in low‐fat fed (LFD, 12% of energy from corn oil) male Wistar rats, and in high‐fat fed (45% of energy from corn oil) obesity‐prone (OP) and obesity‐resistant (OR) male Wistar rats. Designation of OP and OR rats was based on body weight gain (upper tertile for OP; lower tertile for OR) after 5 weeks on the high‐fat diet. Body weight gain over the 5‐week dietary period was 91 ± 9 g in LFD, 98 ± 4 g in OR, and 158 ± 5 g in OP (p<0. 05 vs. LFD and OR). Energy intake over the 5‐week dietary period was 3099 ± 101 kcal in LFD, 3185 ± 51 kcal in OR, and 3728 ± 45 kcal in OP (p<0. 05 vs. LFD and OR). Maximal citrate synthase activity (μ. mol−1 min−1) in the gastrocnemius muscle was not significantly different among groups: 12. 1 ± 2. 4 in LFD, 11. 4 ± 1. 9 in OR and 133 ± 2. 5 in OP rats. Similarly, citrate synthase activity in the heart, 59. 3 ± 7. 2, and liver, 6. 6 ± 0. 4, was also not significantly different among groups. Fatty acid composition of the gastrocnemius muscle was not significantly different among groups. Fasting glycogen levels in the liver, gastrocnemius muscle, and heart were 6. 4 ± 3. 7, 13. 2 ± 2. 3 and 6. 8 ± 1. 9 μmol/g in LFD, 21. 2 ± 5. 1 (p<0. 05 vs. LFD and OP), 10. 4 ± 1. 8 and 5. 9 ± 1. 1 mUmol/g in OR, and 36. 3 ± 4. 8 (p<0. 05 vs. LFD and OR), 10. 2 ± 23 and 53 ± 2. 1 μmol/g in OP rats, respectively. Triglyceride levels were similar among groups in plasma, heart and gastrocnemius muscle, but were significantly (p<0. 05) higher in the liver of OP (15. 5 ± 1. 9 (μmol/g) compared to OR (9. 1 ± 1. 1 μmol/g) and LFD (8. 1 ± 1. 4 μmol/g) rats. These data suggest that susceptibility to dietary obesity, in this rodent model, cannot be explained by differences in tissue oxidative capacity or muscle fatty acid composition.
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In animals, a high-fat diet increases intramuscular fat content, which correlates with both the severity and the temporal development of insulin resistance.
“(iv) in animals, a high fat diet causes an increase in intramuscular fat content, which correlates closely both with the severity and temporal development of insulin resistance [75, 76];”
From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [76]
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References 27 · Cited by 47
- Authors
- Pagliassotti, M. J., Pan, D. A., Prach, P. A., Koppenhafer, T. A., Storlien, L., & Hill, J. O.
- Affiliations
- University of Colorado Health (US)
- Royal Prince Alfred Hospital (AU)
- Corresponding author
- Pagliassotti, Michael J.
- Venue
- Obesity Research
- Publisher
- Wiley
- Published
- 1995-09
- Volume
- 3
- Issue
- 5
- Pages
- 459-464
- ISSN
- 1071-7323 (ISSN-L)
- 1550-8528
- OpenAlex
- W2070123740
- Cited by
- 47
- References
- 27
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2013: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Obesity Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK038088, DK 38088)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- obesity-prone rats, obesity-resistant rats, citrate synthase activity, gastrocnemius, liver glycogen, heart glycogen, high-fat diet, low-fat diet, weight gain, energy intake, oxidative stress, Wistar rats, obesity genetics, mitochondrial function, lipid metabolism, energy metabolism
- MeSH terms
- Adipose Tissue, Animals, Blood Glucose, Body Composition, Energy Intake, Citrate (si)-Synthase, Dietary Fats, Fatty Acids, Glycogen, Insulin, Liver, Male, Myocardium, Obesity, Oxidation-Reduction, Triglycerides, Weight Gain, Rats, Wistar, Muscle, Skeletal, Rats
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