The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
ENZYMIC QUANTIFICATION OF (1→3) (1→4)-β-D-GLUCAN IN BARLEY AND MALT
Open accessAbstract
A simple and quantitative method for the determination of (1→3) (1→4)-β-D-glucan in barley flour and malt is described. The method allows direct analysis of β-glucan in flour and malt slurries. Mixed-linkage β-glucan is specifically depolymerized with a highly purified (1→3) (1→4)-β-D-glucanase (lichenase), from Bacillus subtilis, to tri-, tetra- and higher degree of polymerization (d.p.) oligosaccharides. These oligosaccharides are then specifically and quantitatively hydrolysed to glucose using purified β-D-glucosidase. The glucose is then specifically determined using glucose oxidase/peroxidase reagent. Since barley flours contain only low levels of glucose, and maltosaccharides do not interfere with the assay, removal of low d.p. sugars is not necessary. Blank values are determined for each sample allowing the direct measurement of β-glucan in maltsamples.α-Amylasedoes not interfere with the assay. The method issuitable for the routineanalysis of β-glucan in barley samples derived from breeding programs; 50 samples can be analysed by a single operator in a day. Evaluation of the technique on different days has indicated a mean standard error of 0–1 for barley flour samples containing 3–8 and 4–6% (w/w) β-glucan content.
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ENZYMATIC DETERMINATION OF 1,3:1,4-β-GLUCANS IN BARLEY GRAIN AND OTHER CEREALS†
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“McCleary BV, Glennie-Holmes M. Enzymic quantification of (1→3) (1→4)-βD-glucan in barley and malt.”
MethodLan‐Pidhainy, X., Brummer, Y., Tosh, S. M., Wolever, T. M., & Wood, P. J. (2007) · Cereal Chemistry · doi:10.1094/cchem-84-5-0512 · Cited by 112
Metadata
- Authors
- Mcclear, B. V., & Glennie-Holmes, M.
- Venue
- Journal of the Institute of Brewing
- Published
- 1985-09-10
- Volume
- 91
- Issue
- 5
- Pages
- 285-295
- ISSN
- 0046-9750 (ISSN-L)
- OpenAlex
- W2015331347
- Cited by
- 573
- References
- 11
- FWCI
- 12.70
- Type
- journal article
- Language
- en
- Open access
- gold
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- Mcclear, B. V., & Glennie-Holmes, M. (1985). ENZYMIC QUANTIFICATION OF (1→3) (1→4)-β-D-GLUCAN IN BARLEY AND MALT. Journal of the Institute of Brewing. https://doi.org/10.1002/j.2050-0416.1985.tb04345.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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