The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Novel approach for achieving double‐layered cell sheets co‐culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature‐responsive culture dishes
Abstract
Confluent human aortic endothelial cells (HAECs) cultured on thermo‐responsive culture dish grafted with poly (N‐isopropylacrylamide) were recovered as a contiguous cell sheet. The double‐layered co‐culture was achieved by placing the recovered HAEC sheet onto the rat hepatocyte layer directly. The double‐layered structure of HAEC and hepatocytes remained in tight contact during culture. Hepatocytes in the layered co‐culture system with the HAEC sheet maintained the differentiated cell shape and the albumin expression for over 41 days of culture, whereas the functions disappeared within 10 days of culture in control hepatocytes without the HAEC sheet. The layered co‐culture of hepatocytes and the HAEC sheets, which allows for the expression of differentiated functions of hepatocyte continuously, such as liver lobule, offers a major advancement in liver tissue engineering. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 464–470, 2002
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Bhatia, S. N., Balis, U. J., Yarmush, M. L., & Toner, M. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.14.1883 · Cited by 890
Hepatocytes in collagen sandwich: evidence for transcriptional and translational regulation.
Dunn, J. C., Tompkins, R. G., & Yarmush, M. L. (1992) · The Journal of cell biology · doi:10.1083/jcb.116.4.1043 · Cited by 295
Effect of Collagen Gel Configuration on the Cytoskeleton in Cultured Rat Hepatocytes
Ezzell, R. M., Toner, M., Hendricks, K., Dunn, J. C. Y., Tompkins, R. G., & Yarmush, M. L. (1993) · Experimental Cell Research · doi:10.1006/excr.1993.1266 · Cited by 98
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Edmondson, R., Broglie, J. J., Adcock, A. F., & Yang, L. (2014) · ASSAY and Drug Development Technologies · doi:10.1089/adt.2014.573 · Cited by 2,515
Biomaterials: Where We Have Been and Where We Are Going
Ratner, B. D., & Bryant, S. J. (2004) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.6.040803.140027 · Cited by 1,507
Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets
Ohashi, K., Yokoyama, T., Yamato, M., Kuge, H., Kanehiro, H., Tsutsumi, M., Amanuma, T., Iwata, H., Yang, J., Okano, T., et al. (2007) · Nature Medicine · doi:10.1038/nm1576 · Cited by 507
Self-citationShimizu, T., Sekine, H., Yang, J., Isoi, Y., Yamato, M., Kikuchi, A., Kobayashi, E., & Okano, T. (2006) · The FASEB Journal · doi:10.1096/fj.05-4715fje · Cited by 478
Self-citationTissue Engineering Based on Cell Sheet Technology
Matsuda, N., Shimizu, T., Yamato, M., & Okano, T. (2007) · Advanced Materials · doi:10.1002/adma.200701978 · Cited by 398
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Metadata
- Authors
- Harimoto, M., Yamato, M., Hirose, M., Takahashi, C., Isoi, Y., Kikuchi, A., & Okano, T.
- Venue
- Journal of Biomedical Materials Research
- Publisher
- Wiley
- Published
- 2002-08-22
- Volume
- 62
- Issue
- 3
- Pages
- 464-470
- ISSN
- 0021-9304 (ISSN-L)
- 1097-4636
- OpenAlex
- W2062651358
- Cited by
- 239
- References
- 3
- FWCI
- 11.80
- Type
- journal article
- Language
- en
- Open access
- closed
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