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Cited by 164Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchBone Tissue Engineering Materials

Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells

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Wang, Bo, Borazjani, Ali, Tahai, Mina, de Jongh Curry, Amy L., Simionescu, Dan T., Guan, Jianjun, To, Filip, Elder, Steve H. +1 more·2010·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.32781PMC PMC2933781
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Abstract

Tissue engineered cardiac grafts are a promising therapeutic mode for ventricular wall reconstruction. Recently, it has been found that acellular tissue scaffolds provide natural ultrastructural, mechanical, and compositional cues for recellularization and tissue remodeling. We thus assess the potential of decellularized porcine myocardium as a scaffold for thick cardiac patch tissue engineering. Myocardial sections with 2‐mm thickness were decellularized using 0.1% sodium dodecyl sulfate and then reseeded with differentiated bone marrow mononuclear cells. We found that thorough decellularization could be achieved after 2.5 weeks of treatment. Reseeded cells were found to infiltrate and proliferate in the tissue constructs. Immunohistological staining studies showed that the reseeded cells maintained cardiomyocyte‐like phenotype and possible endothelialization was found in locations close to vasculature channels, indicating angiogenesis potential. Both biaxial and uniaxial mechanical testing showed a stiffer mechanical response of the acellular myocardial scaffolds; however, tissue extensibility and tensile modulus were found to recover in the constructs along with the culture time, as expected from increased cellular content. The cardiac patch that we envision for clinical application will benefit from the natural architecture of myocardial extracellular matrix, which has the potential to promote stem cell differentiation, cardiac regeneration, and angiogenesis. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010

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References 54 · Cited by 164

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Authors
Wang, B., Borazjani, A., Tahai, M., de Jongh Curry, A. L., Simionescu, D. T., Guan, J., To, F., Elder, S. H., & Liao, J.
Affiliations
  • Mississippi State University (US)
  • Clemson University (US)
  • The Ohio State University (US)
Corresponding author
Liao, Jun
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2010-08-02
Volume
94A
Issue
4
Pages
1100-1110
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.32781
PMC
PMC2933781
OpenAlex
W2097356835
Cited by
164
References
54
Citations by year
2026: 4 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 13 · 2020: 9 · 2019: 12 · 2018: 14 · 2017: 8 · 2016: 11 · 2015: 22 · 2014: 10 · 2013: 16 · 2012: 10
FWCI
11.15
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Public Health Service (R1CRH10429-01-00)
  • National Heart, Lung, and Blood Institute (R01HL093399, HL097321, R15HL097321)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
cardiac patch, porcine myocardium, acellular scaffolds, bone marrow mononuclear cells, recellularization, endothelialization, angiogenesis, mechanical properties, extracellular matrix, cardiac regeneration, tissue engineering
MeSH terms
Animals, Bone Marrow Cells, Cells, Cultured, Leukocytes, Mononuclear, Myocardium, Phenotype, Sarcomeres, Staining and Labeling, Porosity, Anisotropy, Tissue Engineering, Sus scrofa, Tissue Scaffolds, Mechanical Phenomena

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