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Cited by 61Bone Tissue Engineering Materialsbiodegradable polymer synthesis and propertiesTissue Engineering and Regenerative Medicine

Composite porous scaffold of PEG/PLA support improved bone matrix deposition in vitro compared to PLA‐only scaffolds

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Bhaskar, Birru, Owen, Robert, Bahmaee, Hossein, Wally, Zena, Sreenivasa Rao, Parcha, Reilly, Gwendolen C.·2018·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.36336
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Abstract

Controllable pore size and architecture are essential properties for tissue‐engineering scaffolds to support cell ingrowth colonization. To investigate the effect of polyethylene glycol (PEG) addition on porosity and bone‐cell behavior, porous polylactic acid (PLA)–PEG scaffolds were developed with varied weight ratios of PLA–PEG (100/0, 90/10, 75/25) using solvent casting and porogen leaching. Sugar 200–300 µm in size was used as a porogen. To assess scaffold suitability for bone tissue engineering, MLO‐A5 murine osteoblast cells were cultured and cell metabolic activity, alkaline phosphatase (ALP) activity and bone‐matrix production determined using (alizarin red S staining for calcium and direct red 80 staining for collagen). It was found that metabolic activity was significantly higher over time on scaffolds containing PEG, ALP activity and mineralized matrix production were also significantly higher on scaffolds containing 25% PEG. Porous architecture and cell distribution and penetration into the scaffold were analyzed using SEM and confocal microscopy, revealing that inclusion of PEG increased pore interconnectivity and therefore cell ingrowth in comparison to pure PLA scaffolds. The results of this study confirmed that PLA–PEG porous scaffolds support mineralizing osteoblasts better than pure PLA scaffolds, indicating they have a high potential for use in bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1334–1340, 2018.

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  • Porogen leaching is the most common method to obtain a porous structure for this type of polymer blend.

    “Porogen leaching is the most common method to obtain a porous structure for this type of polymer blend [178,179].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [178]

    The introduction (p. 4) describes solvent casting–particulate leaching as a widely accepted way to make porous scaffolds, and the conclusion (p. 14) says the study used solvent casting–porogen leaching for PLA–PEG blends. Neither passage establishes that it is the most common method for this type of blend. (LLM, conf=0.86)

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Research works printed but not linked: 25

  1. [6]B. Chen, X. Jing, H. Mi, H. Zhao, W. Zhang, X. Peng, and L. Turng, 'Fabrication of Polylactic Acid/Polyethylene Glycol (PLA/PEG) Porous Scaffold by Supercritical CO2 Foaming and Particle Leaching,' , vol. 55, no. 6, pp. 1339-1348, 2015.
  2. [7]N. Sultana and M. Wang, 'PHBV/PLLA+based composite scaffolds fabricated using an emulsion freezing/freeze+drying technique for bone tissue engineering: surface modification and in vitro biological evaluation,' , vol. 4, no. 1, p. 15003, 2012.
  3. [8]R. Owen, C. Sherborne, T. Paterson, N. H. Green, G. C. Reilly, and F. Claeyssens, 'Emulsion Templated Scaffolds with Tunable Mechanical Properties for Bone Tissue Engineering,' , vol. 54, pp. 159-172, 2015.
  4. [9]K. F. Leong, C. M. Cheah, and C. K. Chua, 'Solid freeform fabrication of three+ dimensional scaffolds for engineering replacement tissues and organs,' , vol. 24, no. 13, pp. 2363-2378, 2003.
  5. [10]T. Serra, M. Ortiz+Hernandez, E. Engel, J. A. Planell, and M. Navarro, 'Relevance of PEG in PLA+based blends for tissue engineering 3D+printed scaffolds,' , vol. 38, no. 1, pp. 55-62, 2014.
  6. [11]N. Thadavirul, P. Pavasant, and P. Supaphol, 'Development of polycaprolactone porous scaffolds by combining solvent casting, particulate leaching, and polymer leaching techniques for bone tissue engineering,' ! " , vol. 102, no. 10, pp. 3379-3392, 2014.
  7. [12]S. Taherkhani and F. Moztarzadeh, 'Fabrication of a poly(ε+caprolactone)/starch nanocomposite scaffold with a solvent+casting/salt+leaching technique for bone tissue engineering applications,' "## , vol. 133, no. 23, pp. 19-21, 2016.
  8. [13]M. Shin, H. Abukawa, M. J. Troulis, and J. P. Vacanti, 'Development of a biodegradable scaffold with interconnected pores by heat fusion and its application to bone tissue engineering,' " , vol. 84A, no. 3, pp. 702-709, 2008.
  9. [14]R. Huang, X. Zhu, T. Zhao, and A. Wan, 'Preparation of tissue engineering porous scaffold with poly(lactic acid) and polyethylene glycol solution blend by solvent+ casting/particulate+leaching,' $# , vol. 1, no. 4, pp. 0-8, 2015.
  10. [15]K. Kim, M. Yu, X. Zong, J. Chiu, D. Fang, Y. S. Seo, B. S. Hsiao, B. Chu, and M. Hadjiargyrou, 'Control of degradation rate and hydrophilicity in electrospun non+ woven poly(D,L+lactide) nanofiber scaffolds for biomedical applications,' , vol. 24, no. 27, pp. 4977-4985, 2003.
  11. [16]Y. Zhang, Z. Wang, F. Jiang, J. Bai, and Z. Wang, 'Effect of miscibility on spherulitic growth rate for double+layer polymer films,' , vol. 9, no. 24, p. 5771, 2013.
  12. [17]Y. Kato, a Boskey, L. Spevak, M. Dallas, M. Hori, and L. F. Bonewald, 'Establishment of an osteoid preosteocyte+like cell MLO+A5 that spontaneously mineralizes in culture.,' , vol. 16, no. 9, pp. 1622-33, 2001.
  13. [18]A. . Stern, M. . Stern, M. . Van Dyke, K. Jähn, M. Prideaux, and L. . Bonewald, 'Isolation and culture of primary osteocytes from the long bones of skeletally mature and aged mice,' % , vol. 52, no. 6, pp. 361-373, 2012.
  14. [19]A. Sittichockechaiwut, A. M. Scutt, A. J. Ryan, L. F. Bonewald, and G. C. Reilly, 'Use of rapidly mineralising osteoblasts and short periods of mechanical loading to accelerate matrix maturation in 3D scaffolds.,' , vol. 44, no. 5, pp. 822-9, May 2009.
  15. [20]R. M. Delaine+Smith, S. MacNeil, and G. C. Reilly, 'Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus,' , vol. 24, no. 0, pp. 162-174, 2012.
  16. [21]M. Sheth, R. A. Kumar, V. Dave, R. A. Gross, and S. P. McCarthy, 'Biodegradable polymer blends of poly(lactic acid) and poly(ethylene glycol),' "## , vol. 66, no. 8, pp. 1495-1505, 1997.
  17. [22]H. Chen, Y. Liu, Z. Jiang, W. Chen, Y. Yu, and Q. Hu, 'Cell+scaffold interaction within engineered tissue,' $# , vol. 323, no. 2, pp. 346-351, 2014.
  18. [23]D. W. Hutmacher, 'Scaffold design and fabrication technologies for engineering tissues++state of the art and future perspectives.,' , vol. 12, no. 1, pp. 107-124, 2001.
  19. [24]J. S. Park, D. G. Woo, B. K. Sun, H. M. Chung, S. J. Im, Y. M. Choi, K. Park, K. M. Huh, and K. H. Park, 'In vitro and in vivo test of PEG/PCL+based hydrogel scaffold for cell delivery application,' , vol. 124, no. 1-2, pp. 51-59, 2007.
  20. [25]X. Zhu, T. Zhong, R. Huang, and A. Wan, 'Preparation of hydrophilic poly(lactic acid) tissue engineering scaffold via (PLA)+(PLA+b+PEG)+(PEG) solution casting and thermal+induced surface structural transformation,' , vol. 26, no. 17, pp. 1286-1296, 2015.
  21. [26]G. Wei and P. X. Ma, 'Macroporous and nanofibrous polymer scaffolds and polymer/bone+like apatite composite scaffolds generated by sugar spheres,' " , vol. 78A, no. 2, pp. 306-315, 2006.
  22. [27]Y. Chen, S. Zhou, and Q. Li, 'Microstructure design of biodegradable scaffold and its effect on tissue regeneration,' , vol. 32, no. 22, pp. 5003-5014, 2011.
  23. [28]F. J. O'Brien, B. A. Harley, I. V. Yannas, and L. J. Gibson, 'The effect of pore size on cell adhesion in collagen+GAG scaffolds,' , vol. 26, no. 4, pp. 433-441, 2005.
  24. [29]R. Dorati, C. Colonna, I. Genta, T. Modena, and B. Conti, 'Effect of porogen on the physico+chemical properties and degradation performance of PLGA scaffolds,' & , vol. 95, no. 4, pp. 694-701, 2010.
  25. [30]T. M. Freyman, I. V. Yannas, and L. J. Gibson, 'Cellular materials as porous scaffolds for tissue engineering,' , vol. 46, no. 3-4, pp. 273-282, 2001.

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Authors
Bhaskar, B., Owen, R., Bahmaee, H., Wally, Z., Sreenivasa Rao, P., & Reilly, G. C.
Affiliations
  • Department of Biotechnology National Institute of Technology Warangal Telangana 506004 India
  • Insigneo (GB)
Corresponding author
Reilly, Gwendolen C.
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2018-01-23
Volume
106
Issue
5
Pages
1334-1340
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.36336
OpenAlex
W2783585064
Cited by
61
References
0
Citations by year
2026: 4 · 2025: 5 · 2024: 12 · 2023: 6 · 2022: 10 · 2021: 5 · 2020: 8 · 2019: 10 · 2018: 1
FWCI
2.34
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
Funders
EPSRC
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
polylactic acid, polyethylene glycol, porous scaffolds, bone tissue engineering, osteoblasts, mineralization, alkaline phosphatase activity, porogen leaching, pore connectivity, cell infiltration, collagen deposition, calcium deposition
MeSH terms
Alkaline Phosphatase, Animals, Bone Matrix, Cell Line, Polyesters, Polyethylene Glycols, Porosity, Mice, Tissue Scaffolds

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