Study of hepatocyte differentiation using embryonic stem cells
Abstract
The liver has many crucial functions including metabolizing dietary molecules, detoxifying compounds, and storing glycogen. The hepatocytes, comprising most of the liver organ, progressively modify their gene expression profile during the fetal development according to their roles in the different phases of development. Embryonic stem (ES) cells serve as a major tool in understanding liver development. These cells may also serve as a source of hepatic cells for cellular therapy. In this review, we aim to summarize the research that has been performed in the field of hepatocyte differentiation from mouse and human ES cells. We discuss the various methodologies for the differentiation of ES cells towards hepatic cells using either spontaneous or directed differentiation protocols. Although many protocols for differentiating ES cells to hepatic cells have been developed, the analysis of their status is not trivial and can lead to various conclusions. Hence, we discuss the issues of analyzing hepatocytes by means of the specificity of the markers for hepatocytes and the status of the cells as fetal or adult hepatocytes. © 2005 Wiley‐Liss, Inc.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, but determining the precise identity of these cells is problematic due to a lack of suitable markers.
“While several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, determining the precise identity of these cells is problematic due to a lack of suitable markers [20-23].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [20]
Cites
Initial sequencing and comparative analysis of the mouse genome
Mouse Genome Sequencing Consortium (2002) · Nature · doi:10.1038/nature01262 · Cited by 7,187 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Hou, J., Charters, A. M., Lee, S. C., Zhao, Y., Wu, M. K., Jones, S. J., Marra, M. A., & Hoodless, P. A. (2007) · BMC Developmental Biology · doi:10.1186/1471-213x-7-92 · Cited by 56 · see citing works
“While several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, determining the precise identity of these cells is problematic due to a lack of suitable markers [20-23].”
BackgroundMicrofluidic platform for hepatitis B viral replication study
Sodunke, T. R., Bouchard, M. J., & Noh, H. (. (2008) · Biomedical Microdevices · doi:10.1007/s10544-007-9148-1 · Cited by 38 · see citing works
Loebel, D. A. F., & Tam, P. P. L. (2005) · Nature Biotechnology · doi:10.1038/nbt1205-1491 · Cited by 18 · see citing works
- Authors
- Lavon, N., & Benvenisty, N.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Benvenisty, Nissim
- ORCID
- Benvenisty, Nissim
- Venue
- Journal of Cellular Biochemistry
- Publisher
- Wiley
- Published
- 2005-10-06
- Volume
- 96
- Issue
- 6
- Pages
- 1193-1202
- ISSN
- 0730-2312 (ISSN-L)
- 1097-4644
- OpenAlex
- W2127333745
- Cited by
- 134
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 8 · 2013: 8 · 2012: 13
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- hepatocyte differentiation, embryonic stem cells, liver development, directed differentiation, spontaneous differentiation, hepatocyte markers
- MeSH terms
- Animals, Cell Differentiation, Embryo, Mammalian, Gene Expression Regulation, Humans, Liver, Liver Diseases, Models, Biological, Stem Cells, Hepatocytes, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
