The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Immunodetection and quantitation of the two forms of transforming growth factor‐beta (TGF‐β1 and TGF‐β2) secreted by cells in culture
Abstract
Transforming growth factor beta (TGF‐β), a potent modulator of cell growth, differentiation, and the expression of extracellular matrix components in a variety of cell types, exists as two distinct homodimers (TGF‐β1 and TGF‐β2), sharing 71% sequence homology. Radioreceptor and previously described radioimmunological assays using rabbit antibodies have not been able to distinguish between these two forms. We have developed antisera in turkeys against native TGF‐β1 and TGF‐β2, each of which specifically blocks both the receptor binding and biological activity of each of these peptides. With these immunological reagents we describe sensitive and specific immunological assays for TGF‐β1 and TGF‐β2 in complex biological fluids. Using these assays we show that both TGF‐β1 and TGF‐β2 are secreted by a variety of cultured cells, but that some cells secrete predominantly either TGF‐β1 or TGF‐β2 while others secrete both peptides in nearly equal amounts. Our results demonstrate that the expression of each of the two forms of TGF‐β is independently regulated.
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Cites
Roberts, A. B., Sporn, M. B., Assoian, R. K., Smith, J. M., Roche, N. S., Wakefield, L. M., Heine, U. I., Liotta, L. A., Falanga, V, & Kehrl, J. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4167 · Cited by 2,866
Self-citationIgnotz, R. A., & Massagué, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35666-1 · Cited by 2,492
Derynck, R., Jarrett, J. A., Chen, E. Y., Eaton, D. H., Bell, J. R., Assoian, R. K., Roberts, A. B., Sporn, M. B., & Goeddel, D. V. (1985) · Nature · doi:10.1038/316701a0 · Cited by 1,667
Self-citationAssoian, R. K., Komoriya, A., Meyers, C. A., Miller, D. M., & Sporn, M. B. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32345-7 · Cited by 1,598
Self-citationMethods in Immunology and Immunochemistry
(1967) · Elsevier eBooks · doi:10.1016/b978-0-12-754401-4.x5001-5 · Cited by 1,133
Type beta transforming growth factor: a bifunctional regulator of cellular growth.
Roberts, A. B., Anzano, M. A., Wakefield, L. M., Roche, N. S., Stern, D. F., & Sporn, M. B. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.1.119 · Cited by 1,094
Self-citationAphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α
IKEGAMI, S., TAGUCHI, T., OHASHI, M., OGURO, M., NAGANO, H., & MANO, Y. (1978) · Nature · doi:10.1038/275458a0 · Cited by 907
Growth Inhibitor from BSC-1 Cells Closely Related to Platelet Type β Transforming Growth Factor
Tucker, R. F., Shipley, G. D., Moses, H. L., & Holley, R. W. (1984) · Science · doi:10.1126/science.6093254 · Cited by 768
Transforming growth factor beta is an important immunomodulatory protein for human B lymphocytes.
Kehrl, J. H., Roberts, A. B., Wakefield, L. M., Jakowlew, S., Sporn, M. B., & Fauci, A. S. (1986) · The Journal of Immunology · doi:10.4049/jimmunol.137.12.3855 · Cited by 759
Self-citationThe transforming growth factor-β system, a complex pattern of cross-reactive ligands and receptors
Cheifetz, S., Weatherbee, J. A., Tsang, M. L.-S., Anderson, J. K., Mole, J. E., Lucas, R., & Massagué, J. (1987) · Cell · doi:10.1016/0092-8674(87)90192-9 · Cited by 706
Cited by
Kulkarni, A. B., Huh, C. G., Becker, D., Geiser, A., Lyght, M., Flanders, K. C., Roberts, A. B., Sporn, M. B., Ward, J. M., & Karlsson, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.2.770 · Cited by 1,967
Self-citationDefective haematopoiesis and vasculogenesis in transforming growth factor-β1 knock out mice
Dickson, M. C., Martin, J. S., Cousins, F. M., Kulkarni, A. B., Karlsson, S., & Akhurst, R. J. (1995) · Development · doi:10.1242/dev.121.6.1845 · Cited by 1,029
Mice lacking Smad3 show accelerated wound healing and an impaired local inflammatory response
Ashcroft, G. S., Yang, X., Glick, A. B., Weinstein, M., Letterio, J. J., Mizel, D. E., Anzano, M., Greenwell-Wild, T., Wahl, S. M., Deng, C., et al. (1999) · Nature Cell Biology · doi:10.1038/12971 · Cited by 911
Self-citationThe Transforming Growth Factor-βs
Roberts, A. B., & Sporn, M. B. (1990) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-642-49295-2_8 · Cited by 746
Self-citationMiller, A., Lider, O., Roberts, A. B., Sporn, M. B., & Weiner, H. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.421 · Cited by 732
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Metadata
- Authors
- Danielpour, D., Dart, L. L., Flanders, K. C., Roberts, A. B., & Sporn, M. B.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1989-01
- Volume
- 138
- Issue
- 1
- Pages
- 79-86
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2064566876
- Cited by
- 444
- References
- 25
- FWCI
- 14.26
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Immunodetection and quantitation of the two forms of transforming growth factor‐beta (TGF‐β1 and TGF‐β2) secreted by cells in culture. https://app.cochat.ai/papers/10.1002/jcp.1041380112
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- Danielpour, D., Dart, L. L., Flanders, K. C., Roberts, A. B., & Sporn, M. B. (1989). Immunodetection and quantitation of the two forms of transforming growth factor‐beta (TGF‐β1 and TGF‐β2) secreted by cells in culture. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.1041380112
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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