Serum Biomarker for Diagnosis of Endometriosis
Abstract
Endometriosis is estimated to affect 10% of women during the reproductive years. The lack of a non‐invasive diagnostic test significantly contributes to the long delay between onset of the symptoms and definitive diagnosis of endometriosis. This case–control study was conducted to identify specific endometriosis antigens using 2D gel analysis in women with endometriosis (n = 5) and without endometriosis (n = 5). Differentially expresses spots were analyzed using matrix‐assisted laser desorption/ionization‐time‐of‐flight/mass spectrometry (nanoLC‐ESI‐MS/MS) with MASCOT analysis, in order to identify the corresponding proteins. ELISAs were performed on a different cohort of endometriosis (n = 120) and healthy patients (n = 20) in order to confirm the differential expression of the identified proteins. ROC analysis of ELISA results confirmed the statistical significance of the differential expression for one of these proteins: Zn‐alpha2‐glycoprotein ( P = 0.019). We propose the analysis of the expression level of this protein in the serum as a new non‐invasive diagnostic test for endometriosis. J. Cell. Physiol. 229: 1731–1735, 2014. © 2014 Wiley Periodicals, Inc.
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Reported sensitivity for Zn-alpha2-glycoprotein was 69.4% and specificity was 100%.
“Reported sensitivity was 69.4% and specificity was 100% [45].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [45]
Zn-alpha2-glycoprotein was identified by mass spectrometry as a possible biomarker for endometriosis and confirmed by ELISA to be differentially expressed (P=0.019) in an additional cohort of endometriosis patients (n=120) and healthy controls (n=20).
“In a study conducted by Signorile and Baldi, Zinc (Zn)-alpha2-glycoprotein was identified by mass spectrometry (endometriosis cases n = 5, controls n = 5) as a possible biomarker for endometriosis and confirmed by ELISA to be differentially expressed (P = 0.019) in an additional cohort of endometriosis patients (n = 120) and healthy controls (n = 20) [45].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [45]
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References 22 · Cited by 35
- Authors
- Signorile, P. G., & Baldi, A.
- Affiliations
- Centro Italiano Endometriosi Rome Italy
- Corresponding author
- Signorile, Pietro Giulio
- Baldi, Alfonso
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 2014-07-29
- Volume
- 229
- Issue
- 11
- Pages
- 1731-1735
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W1572400265
- Cited by
- 35
- References
- 22
- Citations by year
- 2024: 3 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 1
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Cellular Physiology (publishedVersion)
- PubMed (publishedVersion)
- Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli" (submittedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
- Keywords
- endometriosis, serum biomarkers, non-invasive diagnosis, differentially expressed proteins, diagnostic testing
- MeSH terms
- Adult, Endometriosis, Female, Humans, Proteins, ROC Curve, Mass Spectrometry, Electrophoresis, Gel, Two-Dimensional, Reproducibility of Results, Biomarkers, Case-Control Studies, Silver Staining
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